GC-MS分析羌活-独活药对与单味药挥发性成分  

GC-MS analysis of volatile components of Notopterygium incisum-Angelicae pubescentis drug pairs and single drug

作　　者：张育珍 张维迅 吴曼 张博[1] 唐一梅[1] 贺茂芳 张剑 ZHANG Yu-zhen;ZHANG Wei-xun;WU Man;ZHANG Bo;TANG Yi-mei;HE Mao-fang;ZHANG Jian(School of Pharmacy,Xi'an Medical university,Xi'an 710021,China;International College of Pharmaceutical Innovation,Soochow University,suzhou 215222,China)

机构地区：[1]西安医学院药学院,陕西西安710021 [2]苏州大学国际创新药学院,江苏苏州215222

出　　处：《化学研究与应用》2025年第3期735-742,共8页Chemical Research and Application

基　　金：陕西省大学生创新训练计划项目(S202411840058)资助。

摘　　要：为了研究不同配比羌活-独活药对与单味药中挥发油成分差异,采用盐辅助水蒸气蒸馏法提取挥发油,GC-MS分析羌活、独活单味药及羌活-独活药对不同配比挥发性成分,随后利用化学计量学评价挥发性物质成分差异,并进行抗氧化实验。结果表明,通过GC-MS共鉴定出79种挥发性物质,当羌活-独活药对配比为2:1时,得到50种挥发性物质及7种新增化合物。各组挥发性成分差异贡献较大的化合物为间甲酚、(1R)-(+)-α-蒎烯、(R)-(+)-柠檬烯、β-蒎烯和(1S)-(-)-β-蒎烯。羌活、独活及3种不同配比药对具有较好的清除自由基能力。该研究确认了2:1的羌活-独活药对鉴定挥发性物质最多以及导致不同药对配比差异的4种关键化合物,可为羌活-独活药对科学配伍提供参考。To investigate the changes in volatile oil composition between different ratios of Notopterygium incisum-Angelicae pubescentis drug pairs and single drug,salt-assisted water vapor distillation was employed to extract the volatile oils and the volatile components were analyzed using GC-MS.Differences in volatile substance compositions were then evaluated using stoichiometry,and antioxidant experiments were performed.The findings demonstrated that 79 volatile substances in total were detected by GC-MS,and50 volatile substances and 7 novel compounds found when the Notopterygium incisum-Angelicae pubescentis drug pair ratio was 2:1.Compounds such as m-cresol,(1R)-(+)-α-pinene,(R)-(+)-limonene,β-pinene,and(1S)-(-)-β-pinene had a significant role in determining volatile component variations between groups.Notopterygium incisum,Angelicae pubescentis,and three different dosing pairs exhibited superior free radical scavenging activity.In conclusion,the study found the most volatile substances in the 2:1 Notopterygium incisum-Angelicae pubescentis drug pairs,as well as the four essential compounds that caused changes in the ratios of the different pairings,which may be used as a reference for scientific compounding of Notopterygium incisum-Angelicae pubescentis drug pairs.

关 键 词：羌活-独活药对 挥发油 抗氧化 

分 类 号：O657.7[理学—分析化学]