乌鳢源鰤鱼诺卡氏菌分离、鉴定及致病性分析  

Isolation,identification and pathogenicity analysis of Nocardia seriolae from Channa argus

作　　者：李娜 唐怀庆 丘金珠 宋长江 夏立群[1,2] 鲁义善 夏洪丽[1,2,4] 喻大鹏 LI Na;TANG Huaiqing;QIU Jinzhu;SONG Changjiang;XIA Liqun;LU Yishan;XIA Hongli;YU Dapeng(School of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China;Shenzhen Research Institute of Guangdong Ocean University,Shenzhen 518116,China;Zhongshan Institute for Quality and Safety Inspection of Agricultural Products,Zhongshan 528400,China;Shenzhen Yihai Biotechnology Co.,Ltd.,Shenzhen 518116,China)

机构地区：[1]广东海洋大学水产学院,广东湛江524088 [2]广东海洋大学深圳研究院,广东深圳518116 [3]中山市农产品质量安全检验所,广东中山528400 [4]深圳义海生物科技有限公司,广东深圳518116

出　　处：《江苏海洋大学学报(自然科学版)》2025年第1期1-7,共7页Journal of Jiangsu Ocean University:Natural Science Edition

基　　金：国家重点研发计划项目(2020YFD0900201);中山市第二批社会公益与基础研究项目(2021B2058)。

摘　　要：为了解中山市某水产养殖基地乌鳢爆发性死亡病因,从患病乌鳢脾脏、肝脏、体肾等病灶组织分离得到一株优势菌株,命名为ZS0721。对分离菌株进行人工感染实验,确认引起乌鳢爆发性死亡致病菌为ZS0721。最后对分离菌株进行形态学特征观察、生理生化特性鉴定、药敏分析与组织病理切片分析。结果显示,菌株ZS0721具有较强致病性,对乌鳢的半致死浓度LD_(50)为2.87×10^(6) CFU/mL;其16S rDNA序列和NCBI数据库中鰤鱼诺卡氏菌(Nocardia seriolae)MT773631.1具有99.38%同源性;且该菌株的生理生化特性与鰤鱼诺卡氏菌也相一致。综合上述结果,确定该菌株为鰤鱼诺卡氏菌(N. seriolae)。组织病理学分析表明,菌株ZS0721感染引起了鱼体免疫组织脾脏、肝脏和体肾出现肉芽肿病变。药敏实验结果显示,该菌株对头孢哌酮、链霉素、卡那霉素、庆大霉素、大观霉素、四环素等13种抗生素敏感。该研究为乌鳢鰤鱼诺卡氏菌感染防控提供一定参考依据。In order to understand the cause of the explosive death of Channa argus in an aquaculture base in Zhongshan city,a dominant strain was isolated from diseased Channa argus spleen,liver,kidney,named ZS0721.Subsequently,the artificial infection experiment caused the explosive death of Channa argus was confirmed as ZSo721.Finally,the morphological characteristics observation,physiological and biochemical characterization,drug sensitivity analysis and histopathological sections were analyzed.The results showed that strain ZS0721 was highly pathogenic,with a semi-lethal concentration of LD_(50) of 2.87×10^(6) CFU/mL;the 16S rDNA sequence was similiary with the strain Nocardia seriolae MT773631.1 with 99.38%homology in the NCBI database;and the physiological and biochemical characteristics of this strain were consistent with those of N.seriolae.Based on the above results,the strain was identified as N.seriolae.Histopathological analysis indicated that strain zso721 infection caused granulomatous lesions in the spleen,liver,and kidney of fish immune tissues.The results of the drug susceptibility experiment showed that the strain was sensitive to thirteen antibiotics including cefoperazone,streptomycin,kanamycin,gentamycin,spectinomycin,and tetracycline.This study provides a reference for the control of N.seriolae infection in Channa argus。

关 键 词：乌鳢 鰤鱼诺卡氏菌 药敏 组织病理 

分 类 号：S941.42[农业科学—水产养殖]