Box-Behnken响应面法优化地榆多糖的提取工艺  

Optimize the extraction process of polysaccharide from Sanguisorba officinalis L.by Box-Behnken response surface method

作　　者：吴丽红[1,2] 杨欣荣 刘运伟 杨春娟[3] 王知斌[1] WU Li-hong;YANG Xin-rong;LIU Yun-wei;YANG Chun-juan;WANG Zhi-bin(Heilongjiang University of Chinese Medicine,Harbin 150040,China;Post-Doctoral Mobile Station of Heilongjiang University of Chinese Medicine,Harbin 150040,China;Harbin Medical University,Harbin 150081,China;Yichun Branch of Heilongjiang Academy of Forestry Sciences,Yichun 153000,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江中医药大学博士后流动站,黑龙江哈尔滨150040 [3]哈尔滨医科大学,黑龙江哈尔滨150081 [4]黑龙江省林业科学院伊春分院,黑龙江伊春153000

出　　处：《化学工程师》2025年第3期83-86,55,共5页Chemical Engineer

基　　金：国家自然科学基金资助面上项目(81573551);黑龙江省重点研发计划项目(GA22B012);黑龙江省中医药管理局科研课题(ZHY2024-049);黑龙江省省属基本科研业务费项目(CZKYF2023-1-B019)。

摘　　要：目的确定地榆多糖的最佳提取工艺条件。方法采用苯酚-硫酸法,在490nm波长处测定吸光度,以地榆多糖得率为指标,通过单因素考察和Box-Behnken响应面法实验优化地榆多糖的提取工艺。结果地榆多糖的最佳提取工艺为:提取液料比为17(mL∶g)、回流时间为127min、回流次数为3次,此条件下的地榆多糖得率为1.93%。结论Box-Behnken响应面法优化地榆多糖的提取工艺方法具有准确性和可靠性,提取工艺条件合理可行。OBJECTIVE To optimize the extraction conditions for polysaccharide from Sanguisorba officinalis L.METHODS The phenol-sulfuric acid method was employed to determine the absorbance at a wavelength of 490 nm.The extraction process of polysaccharide from Sanguisorba officinalis L.was optimized through single factor investigation and the Box-Behnken response surface method.RESULTS The optimal extraction process was conducted as follows:the ratio of extraction material to liquid was 1∶17(g∶mL),the extraction duration lasted for 127 minutes,and the number of extractions performed was 3 times.Under these specified conditions,the yield of polysaccharide reached 1.93%.CONCLUTION The Box-Behnken response surface method is highly accurate and reliable,while the extraction conditions are deemed reasonable and feasible.

关 键 词：地榆 多糖 提取工艺 Box-Behnken响应面法 

分 类 号：R284.2[医药卫生—中药学]