从印度尼西亚进口玉米中首次截获玉米褪绿斑驳病毒  

Interception of maize chlorotic mottle virus on imported corn from Indonesia for the first time

作　　者：单振菊 水克娟 闫邦奇 刘明航 徐淼锋 廖力 张卫东 Shan Zhenju;Shui Kejuan;Yan Bangqi;Liu Minghang;Xu Miaofeng;Liao Li;Zhang Weidong(Gongbei Customs District Technical Center,Zhuhai 519000,China;Zhongshan Customs House Technical Center;Guangzhou Customs District Technical Center)

机构地区：[1]拱北海关技术中心,广东珠海519000 [2]中山海关技术中心 [3]广州海关技术中心

出　　处：《植物检疫》2025年第2期63-66,共4页Plant Quarantine

基　　金：拱北海关科研项目(2023GK008,2023GK006);科技部科技基础资源调查专项(2023FY100402)。

摘　　要：玉米褪绿斑驳病毒(maize chlorotic mottle virus,MCMV)是我国进境植物检疫性有害生物。主要分布于秘鲁、美国、墨西哥、刚果民主共和国、肯尼亚、阿根廷、巴西等国家,中国台湾、四川和云南曾有过报道。2024年6月拱北海关技术中心通过双抗体夹心酶联免疫吸附(DAS-ELISA)、RT-PCR方法、实时荧光RT-PCR和序列比对分析进行验证,首次从印度尼西亚进口玉米种子中检出该病毒。这是我国首次从印度尼西亚进口玉米种子中截获该病毒,可为海关一线人员执法把关提供技术支撑。Maize chlorotic mottle virus(MCMV)is a quarantine pest for imported plants in China.It is mainly distributed in countries such as Peru,the United States,Mexico,the Democratic Republic of the Congo,Kenya,Argentina,and Brazil.It has been reported in Taiwan,Sichuan and Yunnan in China.In June,2024,the Technical Center of Gongbei Customs detected and verified this virus in maize seeds imported from Indonesia for the first time through these methods such as DAS-ELISA,RT-PCR,real-time fluorescent RT-PCR,and sequence alignment analysis.This was the first interception of MCMV in maize seeds imported from Indonesia in China,which can provide technical support for the law enforcement and inspection work of frontline customs officers.

关 键 词：玉米褪绿斑驳病毒 DAS-ELISA RT-PCR 实时荧光RT-PCR 序列分析 

分 类 号：S41-31[农业科学—植物保护]