高效液相色谱法测定防腐材中嘧菌酯含量的方法研究  

Measurement of the Azoxystrobin Content in Preservative-Treated Wood by High Performance Liquid Chromatography

作　　者：张景朋 邵闯 蒋明亮[1] ZHANG Jingpeng;SHAO Chuang;JIANG Mingliang(Research Institute of Wood Industry,Chinese Academy of Forestry,Beijing 100091,China)

机构地区：[1]中国林业科学研究院木材工业研究所,北京100091

出　　处：《木材科学与技术》2025年第1期64-70,共7页Chinese Journal of Wood Science and Technology

基　　金：国家自然科学基金资助项目“木质素负载IPBC纳米微球载药体系的构建及对木材防霉性能的增效机制”(32301525)。

摘　　要：为实现防腐处理材中嘧菌酯(azoxystrobin)含量的准确快捷检测,首先建立嘧菌酯的高效液相色谱(HPLC)分析方法(紫外检测器及C18反相色谱柱,V(甲醇)∶V(水)=3∶1为流动相,流速0.8 mL/min,检测波长220 nm),然后利用超声波提取木/竹材中的防腐剂,并优化提取的条件。结果表明:嘧菌酯在质量浓度10~120 mg/L的范围内,标准曲线的决定系数为0.9997,线性相关性显著,分析样品的变异系数为1.39%和1.55%,样品的回收率为96.5%~101.6%。在优化的超声波提取条件下:溶剂配比为v(甲醇)∶v(水)=8∶2,提取时间60 min,2个载药量水平,辐射松(Pinus radiata)、马尾松(Pinus massoniana)、杉木(Cunninghamia lanceolata)、和107杨(Populus×euramericana cv.‘74/76’)防腐木中嘧菌酯的回收率为95.5%~99.6%,标准偏差在0.8%~3.8%范围内;毛竹(Phyllostachys edulis)提取回收率为84.4%和88.3%。本方法可用于检测处理材中嘧菌酯的含量,前处理步骤简便快捷,分析结果准确度高,重现性好。In order to measure the content of azoxystrobin in treated wood precisely,a high performance liquid chromatography(HPLC)analysis method was constructed using a UV detector and C18 reversedphase chromatography column,the V(methanol)∶V(water)=3∶1 as the mobile phase,with a flow rate of 0.8 mL/min and UV detection wavelength of 220 nm.Then,the ultrasonic extraction was used for pretreatment of preservative extraction in wood/bamboo,and the extraction conditions were optimized.The results showed that the determination coefficient of the standard curve for azoxystrobin was 0.9997 within the mass concentration range of 10~120 mg/L,and the linear correlation was significant.The coefficients of variation were 1.39%and 1.55%,and the recovery rates were 96.5%~101.6%.Under the optimized ultrasonic extraction conditions,including the solvent ratio of v(methanol)∶v(water)=8∶2,the extraction time of 60 minutes,and two retention levels,the azoxystrobin recovery rate of Radiata pine,masson pine,Chinese fir,and poplar‘107’was 95.5%~99.6%,with a standard deviation of 0.8%~3.8%.The recovery rates of Moso bamboo extraction were 84.4%and 88.3%,respectively.This method can be used to measure the azoxystrobin content in treated materials,with simple and fast pretreatment steps,achieving a high precision of analysis results and the good reproducibility.

关 键 词：嘧菌酯 超声波提取 高效液相色谱 防腐材 

分 类 号：S782.33[农业科学—木材科学与技术]