PTH(1-34)诱导骨矿化小鼠实验动物模型的构建及鉴定  

Construction and identification of PTH(1-34)-induced bone mineralization experimental animal model in mice

作　　者：王红丽 李晓青 WANG Hongli;LI Xiaoqing(Department of Biochemistry and Molecular Biology,Tianjin Medical University Cancer Institute and Hospital,Tianjin Medical University Cancer Institute&Hospital,National Clinical Research Center for Cancer,Tianjin′s Clinical Research Center for Cancer,Key Laboratory of Cancer Prevention and Therapy,Tianjin,Key Laboratory of Breast Cancer Prevention and Therapy,Tianjin Medical University,Ministry of Education,Tianjin 300060,China)

机构地区：[1]天津医科大学肿瘤医院肿瘤研究所生物化学与分子生物学研究室,国家恶性肿瘤临床医学研究中心,天津市恶性肿瘤临床研究中心,天津市肿瘤防治重点实验室,乳腺癌防治教育部重点实验室,天津300060

出　　处：《天津医科大学学报》2025年第2期134-138,共5页Journal of Tianjin Medical University

基　　金：天津市医学重点科学(专科)建设项目(TJYXZDXK-009A)。

摘　　要：目的:探讨骨骼发育及矿化过程,构建骨矿化小鼠实验动物模型。方法:采用甲状旁腺激素(PTH)(1-34)经腹腔注射,诱导6周龄NOD-SCID雌性小鼠成骨细胞分化及骨组织矿化,从而构建骨矿化小鼠实验动物模型。进一步应用Micro-CT检测骨密度,应用钙黄绿素和盐酸四环素荧光标记检测骨矿化沉积率,应用冯库萨染色鉴定钙盐沉积,并应用马松染色检测骨胶原容积分数,以鉴定小鼠骨组织矿化水平,确定骨矿化小鼠模型是否构建成功。结果:Micro-CT检测发现,PTH(1-34)诱导20 d可以显著增加骨小梁相对体积分数(BV/TV,t=9.473,P<0.001)和骨小梁厚度(Tb.Th,t=18.64,P<0.001),显著减少骨小梁间距(Tb.Sp,t=7.794,P<0.01)。双荧光标记显示,PTH(1-34)处理后骨矿化沉积率(MAR)显著提高(t=29.99,P<0.001)。冯库萨染色显示,PTH(1-34)处理组冯库萨阳性面积(VK.A)显著增加(t=2.914,P<0.05)。马松染色显示,PTH(1-34)处理后胶原容积分数(CVF)显著提升(t=13.94,P<0.001)。结论:PTH(1-34)可有效诱导小鼠骨骼矿化,成功构建骨矿化小鼠实验动物模型。Objective:To investigate the process of bone development and mineralization,and construct an experimental animal model of bone mineralization in mice.Methods:PTH(1-34)(parathyroid hormone 1-34)was injected intraperitoneally to induce osteoblast differentiation and bone tissue mineralization in 6-week-old NOD-SCID female mice,and an experimental animal model of bone mineralization was constructed.Furthermore,micro-CT was used to detect bone mineral density,calcein and tetracycline hydrochloride fluorescent labeling was used to detect mineral apposition rate.Von Kossa staining was used to identify calcium salt deposition,and Masson staining was used to detect bone collagen volume fraction to identify the level of bone tissue mineralization in mice and determine whether the mouse model of bone mineralization was successfully established.Results:Micro-CT showed that PTH(1-34)induction for 20 days significantly increased the Bone Volume/Total Volume(BV/TV,t=9.473,P<0.001)and trabecular thickness(Tb.Th)(t=18.64,P<0.001),while significantly reduced trabecular spacing(Tb.Sp,t=7.794,P<0.01).The double fluorescent labeling showed that the mineral appo-sition rate(MAR)of bone mineralization was significantly increased after PTH(1-34)treatment(t=29.99,P<0.001).Von Kossa staining showed that the Von Kossa Area(VK.A)was significantly increased in the PTH(1-34)treatment group(t=2.914,P<0.05).Mason-ing showed that the collagen volume fraction(CVF)was significantly increased after PTH(1-34)treatment(t=13.94,P<0.001).Conclusion:PTH(1-34)can effectively induce bone mineralization in mice,and successfully construct an experimental animal model of bone mineralization in mice.

关 键 词：甲状旁腺激素(1-34) 骨矿化 骨密度 实验动物模型 

分 类 号：R589.5[医药卫生—内分泌]