苏州地区汉族人群MNS血型系统基因频率及多态性研究  

Gene frequencies and polymorphism of the MNS blood group system in the Han population of voluntary blood donors in Suzhou

作　　者：徐子豪 傅晓燕[2] 刘贞 蒋佳 金一鸣[1] XU Zihao;FU Xiaoyan;LIU Zhen;JIANG Jia;JIN Yiming(Suzhou Blood Center,Suzhou 215000,China;Tai Cang Blood Branch Station,Taicang 215400,China)

机构地区：[1]苏州市中心血站,江苏苏州215000 [2]苏州市中心血站太仓分站,江苏太仓215400

出　　处：《中国输血杂志》2025年第3期397-401,425,共6页Chinese Journal of Blood Transfusion

基　　金：苏州市卫生科技项目(GWZX202203);姑苏卫生人才科研项目(GSWS2023032);苏州市医学重点学科(SZXK202118);苏州市科技计划项目(SKYD2022042);江苏省输血协会爱康生物科研基金项(JSAK2022005)

摘　　要：目的了解苏州地区汉族献血者人群MNS血型系统抗原和基因频率分布及稀有表型的基因多态性,以完善本地区稀有血型库的建设。方法随机收集2023年10月-2024年6月在本站献血的汉族献血者的全血标本8034份,以血清学方法鉴定标本MNS血型表型,分析基因频率并与其他地区民族人群比较,对MNS稀有表型标本进行基因测序。结果本研究人群MNS血型系统中,MM、NN、MN表型分别占23.00%、27.12%、49.88%;SS、ss、Ss表型分别占0.30%、90.99%、8.70%;M、N、S、s的基因频率分别为0.4794、0.5206、0.0465、0.9534,经χ^(2)检验分析均符合Hardy-Weinberg平衡法则,P值分别为0.997、0.349,与部分其他地区民族人群相比有差异(P<0.05)。同时还发现1例罕见血清学表型S-s-,频率为0.01%。结论苏州地区汉族献血者人群MNS血型具有多态性和地域性分布的特点,基因频率与我国其他地区有差异,应进一步加强建设苏州地区稀有血型库,为临床精准用血提供数据支持。Objective To investigate the antigen and gene frequency distribution of the MNS blood group system in the Han population of voluntary blood donors in Suzhou,and to explore the polymorphism of rare MNS blood group genes,in or-der to improve the construction of the local rare blood group database.Methods A total of 8034 whole blood samples were randomly collected from Han blood donors at our station from October 2023 to June 2024.The MNS blood group phenotypes were identified using serological methods.Gene frequencies were analyzed and compared with those of ethnic populations in other regions.Rare MNS phenotype samples were subjected to gene sequencing.Results The distribution of MNS blood group system phenotypes within the population was as follows:the MM,NN,and MN phenotypes accounted for 23.00%,27.12%,and 49.88%respectively;the SS,ss,and Ss phenotypes accounted for 0.30%,90.99%,and 8.70%respec-tively.The gene frequencies of M,N,S,and s were 0.4794,0.5206,0.0465,and 0.9534 respectively.Chi-squared tests confirmed adherence to Hardy-Weinberg equilibrium with P-values of 0.997 and 0.349,showing statistical significance compared to some other regional ethnic populations(P<0.05).Additionally,one rare serological phenotype,S-s-,with a frequency of 0.01%,was identified.Conclusion The MNS blood group system in the Han population of voluntary blood donors in Suzhou exhibits polymorphism and regional distribution characteristics.Gene frequencies differ from those ob-served in other regions of China.It is essential to enhance the establishment of a rare blood type database in Suzhou to pro-vide data support for precise clinical transfusion.

关 键 词：MNS血型系统 稀有血型 基因频率 基因多态性 

分 类 号：R457.11[医药卫生—治疗学]