血小板添加液ⅢM悬浮的血小板保存期质量的研究  

Platelet quality during storage of platelet concentrates in Platelet Addictive SolutionⅢM:a comparative study

作　　者：刘宇健[1] 游冉冉[1] 林绮文[1] 何博[1] 聂咏梅[1] LIU Yujian;YOU Ranran;LIN Qiwen;HE Bo;NIE Yongmei(Guangzhou Blood Center,Guangzhou 510095,China)

机构地区：[1]广州血液中心,广东广州510095

出　　处：《中国输血杂志》2025年第3期408-414,共7页Chinese Journal of Blood Transfusion

基　　金：广州市科技计划项目(2023A03J0544)。

摘　　要：目的 采用血小板添加液ⅢM作为介质悬浮浓缩血小板,检测不同保存期血小板的质量,并探究保存浓缩血小板的介质中血小板添加液ⅢM与血浆的合适比例。方法 采用带有血小板保存袋的一次性使用塑料血袋,采集健康无偿献血者全血,用富浆法制备收集浓缩血小板,容量约50 mL,每袋所含血小板≥4.0×10~(10)个。分别采用不同比例的血小板添加液ⅢM与血浆组成的介质来悬浮血小板。根据悬浮血小板的介质中血小板添加液ⅢM∶血浆体积比的不同,将血小板分为3组:对照组(采用血浆介质悬浮血小板)、试验1组(采用血小板添加液ⅢM∶血浆体积比为6.5∶3.5的介质悬浮血小板)和试验2组(低血浆组,采用血小板添加液ⅢM∶血浆体积比为9∶1的介质悬浮血小板),每组标本量为50例。将不同介质悬浮的3组血小板置于(22±2)℃振荡保存,分别在保存期d1、3、5、7采用无菌接驳取样5 mL,检测血小板计数、pH值、乳酸脱氢酶、CD62P阳性率及Annexin V阳性率。应用SPSS24.0统计软件分析上述检测所得参数,采用单因素方差分析,多个样本均数间的两两比较用Bonferroni法,比较3组参数的差异。结果 随保存时间延长,3组血小板计数均下降、Annexin V阳性率均升高,3组差异不具统计学意义(P>0.05);3组pH值下降,在d1、3、5、7分别为7.44±0.13 vs 7.44±0.14 vs 7.41±0.11、7.31±0.68 vs 7.43±0.23 vs 7.22±0.12、7.30±0.15 vs 7.42±0.14 vs 7.17±0.12、7.29±0.33 vs 7.26±0.18 vs 7.04±0.12,对照组和试验1组pH值下降幅度小,差异不具统计学意义,试验2组在d5、7下降幅度较大,差异具有统计学意义(P<0.05);3组乳酸脱氢酶均升高(mmol/L),在d1、3、5、7分别为169.62±99.33 vs 105.80±150.71 vs 77.14±105.38、225.10±112.86 vs 116.00±72.77 vs 94.42±88.74、249.42±79.55 vs 119.00±53.51 vs 118.35±80.39、253.34±86.95 vs 147.71±90.71 vs 124.68±128.68,与对照组相比,差异具有统计学意义(P<0.05)Objective To use Platelet Additive SolutionⅢM to suspend concentrated platelets and evaluate their qual-ity at different storage periods,in order to investigate the optimal ratio ofⅢM to plasma in the medium for storing concen-trated platelets.Methods Disposable plastic blood bags with platelet storage bags were used to collect whole blood from healthy voluntary blood donors,and concentrated platelets were collected by plasma-rich method,with a volume of about 50 mL and≥4.0×1010 platelets contained in each bag.According to the Platelet Addictive SolutionⅢM/plasma volume ratio in the medium of suspended platelets,the platelets were divided into 3 groups:control group(plasma only),experimental group 1(Platelet Addictive SolutionⅢM/plasma volume ratio of 6.5∶3.5)and experimental group 2(low plasma group,Platelet Addictive SolutionⅢM/plasma volume ratio of 9∶1),each group of 50 samples.Three groups of platelets were stored at(22±2)℃at a flat-bed shaker,and 5 mL were sampled by sterile connection at day 1,3,5 and 7 respectively to detect platelet count,pH value,lactate dehydrogenase,CD62P positive rate and Annexin V positive rate.All the data were analyzed with SPSS24.0 software.One-way ANOVA was employed to compare the differences among three groups.In order to pairwise comparisons between means of multiple samples,Bonferroni method was applied.Results With the extension of storage time,the platelet count decreased and the Annexin V positive rate increased in the 3 groups,and the difference of the 3 groups was not statistically significant(P>0.05).The pH value decreased in the 3 groups,with values at day 1,3,5 and 7 of 7.44±0.13 vs 7.44±0.14 vs 7.41±0.11,7.31±0.68 vs 7.43±0.23 vs 7.22±0.12,7.30±0.15 vs 7.42±0.14 vs 7.17±0.12,7.29±0.33 vs 7.26±0.18 vs 7.04±0.12,respectively.The pH decline in the control group and experi-ment group 1 was minor,with no statistically significant difference,but experiment group 2 showed relatively larger decrea-ses in day 5 and day 7,with statistically sign

关 键 词：血小板保存液ⅢM 血小板质量 CD62P阳性率 

分 类 号：R331.143[医药卫生—人体生理学]