CRISPR技术在链霉菌细胞工厂中的应用和优化  

Application and optimization of CRISPR editing technology in Streptomyces

作　　者：李新颖 苏畅 郭超 庞建 王超 李春 LI Xinying;SU Chang;GUO Chao;PANG Jian;WANG Chao;LI Chun(Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering,Ministry of Industry and Information Technology,Institute of Biochemical Engineering,School of Chemistry and Chemical Engineering,Beijing Institute of Technology,Beijing 100081,China;Academy of National Food and Strategic Reserves Administration,Institute of Cereal&Oil Science and Technology,Beijing 100037,China;Center for Synthetic&Systems Biology,Department of Chemical Engineering,Tsinghua University,Beijing 100084,China)

机构地区：[1]北京理工大学化学与化工学院生物化工研究所/医药分子科学与制剂工程工信部重点实验室,北京100081 [2]国家粮食和物资储备局科学研究院,粮油加工研究所,北京100037 [3]清华大学化学工程系/生物化工研究所,北京100084

出　　处：《化工学报》2025年第3期922-932,共11页CIESC Journal

基　　金：国家自然科学基金项目(22108153);十四五重点研发计划项目(2023YFA0914700);工业生物催化教育部重点实验室(清华大学)开放基金项目(2023003)。

摘　　要：链霉菌底盘细胞的开发是微生物源药物高效合成的有效策略,高效的基因编辑工具为构建链霉菌细胞工厂提供了有力的技术支撑。CRISPR(clustered regularly interspaced short palindromic repeats)技术具有精准性、普适性和易操作性,在微生物中得到了广泛的应用。然而脱靶效应、Cas蛋白毒性、编辑效率低下等原因,导致CRISPR在链霉菌中的应用受到一定的限制。对CRISPR相关技术在链霉菌中的应用及优化策略进行了总结和归纳,并对CRISPR系统在链霉菌细胞工厂中的应用前景进行了展望,为开发适用于链霉菌的高效基因编辑工具提供了参考。Streptomyces chassis development is an effective strategy for the high-efficient synthesis of microbial source nature product,and highly efficient gene editing tools provide a powerful tool for the construction of Streptomyces cell factories.CRISPR(clustered regularly interspaced short palindromic repeats)technology is accurate,universal and easy to operate,and has been widely used in microorganisms.However,off-target effects,cytotoxicity and low editing efficiency limit application of CRISPR genome editing in Streptomyces.This article summarizes the application and optimization strategies of CRISPR-related technologies in Streptomyces,and looks forward to the application prospects of CRISPR systems in Streptomyces cell factories,providing a reference for the development of efficient gene editing tools suitable for Streptomyces.

关 键 词：CRISPR 链霉菌 基因编辑 生物技术 优化策略 

分 类 号：Q812[生物学—生物工程]