亚麻GRAS基因家族的全基因组分析及其对外源赤霉素的响应研究  

Whole Genome Analysis of the GRAS Gene Family in Flax and its Response to Exogenous Gibberellin

作　　者：李静 华鑫涛 杨芸 鲁若妍 刘畅 孙健 戴志刚[2] 谢冬微 LI Jing;HUA Xintao;YANG Yun;LU Ruoyan;LIU Chang;SUN Jian;DAI Zhigang;XIE Dongwei(School of Life Sciences,Nantong University,Nantong 226019,Jiangsu,China;Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha 410221,Hunan,China)

机构地区：[1]南通大学生命科学学院,江苏南通226019 [2]中国农业科学院麻类研究所,湖南长沙410221

出　　处：《中国麻业科学》2025年第1期1-13,共13页Plant Fiber Sciences in China

基　　金：国家自然科学基金青年项目(31801409);国家自然科学基金面上项目(32472174)。

摘　　要：亚麻(Linum usitatissimum L.)是重要的纤用作物,GRAS基因家族在植物生长发育中具有重要作用。研究利用生物信息学从亚麻全基因组数据库鉴定LuGRAS蛋白序列,经SMART和Pfam-search去除冗余序列后,通过ExPASy-ProParam、TBtools以及MEGA7.0等进行理化性质、染色体定位和系统进化等分析,结合转录组测序(RNA-seq)分析亚麻GRAS家族成员在不同组织、不同时期的表达模式,并用实时荧光定量PCR(qRT-PCR)技术检测部分具有显著差异的LuGRAS基因在赤霉素处理后的表达情况,通过共表达网络对LuGRAS基因之间的关系进行分析。结果表明:从亚麻全基因组中鉴定出114个LuGRAS转录因子,根据亲缘关系远近分为10个亚族;亚麻GRAS蛋白氨基酸数介于135~1377个,分子量介于15.3~152.9 kDa,平均等电点5.96,除LuGRAS114外,其他LuGRAS基因分布在15条染色体上;基于亚麻RNA-seq数据,可以将LuGRAS基因分为7组;通过qRT-PCR验证8个表达量具有显著差异的LuGRAS基因,结果显示,有4个LuGRAS基因经赤霉素处理后不同时间段的表达量变化明显。LuGRAS50与LuGRAS94的表达模式表现出极高的正相关关系,LuGRAS38和LuGRAS50与LuGRAS94之间可能存在负调控关系。以上结果将为进一步研究LuGRAS基因的生物学功能及其在亚麻生长发育中的表达模式提供理论参考。Flax is an important fiber crop,and the GRAS gene family plays a crucial role in plant growth and development.In this study,bioinformatics was used to identify LuGRAS protein sequences 1 from the whole flax genome database.After removing redundant sequences by SMART and Pfam search,physical and chemical properties,chromosome localization and systematic evolution were analyzed using ExPASy ProParam,TBtools and MEGA7.0.In combination with transcriptome sequencing(RNA-seq),the expression patterns of flax GRAS family members in different tissues and stages were analyzed.Realtime fluorescence quantitative PCR(qRT-PCR)technology was used to detect the expression of some LuGRAS genes with significant differences after gibberellin treatment.The relationship between LuGRAS genes was analyzed by co-expression network.The results showed that 114 LuGRAS transcription factors were identified from the whole flax genome,which were divided into 10 subgroups based on their genetic relatedness.The number of amino acids in the GRAS proteins of flax ranges from 135 to 1377,with a molecular weight of 15.3—152.9 kDa and an average isoelectric point of 5.96.Except for LuGRAS114,the other LuGRAS genes are distributed on 15 chromosomes;Based on the RNA seq data of flax,the LuGRAS genes can be categorized into 7 groups;By qRT-PCR verification,8 LuGRAS genes with significant differences in expression were identified.The results showed that 4 LuGRAS genes exhibited significant changes in expression levels at different time points after treatment with gibberellin.The expression patterns of LuGRAS50 and LuGRAS94 show a strong positive correlation.There is a negative regulatory relationship between LuGRAS38,LuGRAS50 and LuGRAS94.The above results provide a theoretical reference for further studying the biological function of the LuGRAS genes in flax and their expression patterns during growth and development of flax.

关 键 词：亚麻 GRAS基因家族 全基因组 生物信息学分析 表达分析 

分 类 号：S563.2[农业科学—作物学]