工业大麻中大麻二酚酸(CBDA)含量测定方法优化及光质调控分析  

Optimization of Extraction Method for Cannabidiol Acid(CBDA)from Industrial Hemp and Light Quality Control Analysis

作　　者：石雨[1,2] 李国巍 张婧如[1,2] 田媛 张治国[1,2] 高宝昌[1,2] SHI Yu;LI Guowei;ZHANG Jingru;TIAN Yuan;ZHANG Zhiguo;GAO Baochang(Daqing Branch of Heilongjiang Academy of Sciences,Daqing 163319,Heilongjiang,China;Engineering Research Center for Hemp and Product in Cold Region of Ministry of Education,Qiqihar 161006,Heilongjiang,China)

机构地区：[1]黑龙江省科学院大庆分院,黑龙江大庆163319 [2]寒区麻及制品教育部工程研究中心,黑龙江齐齐哈尔161006

出　　处：《中国麻业科学》2025年第1期24-35,共12页Plant Fiber Sciences in China

基　　金：黑龙江省科学院青年创新基金项目(CXMS2023DQ02);黑龙江省科学院双提雁阵特别计划项目(YZQY2023DQ01);黑龙江省省属科研院所科研业务费项目(CZKYF2023-1-B042)。

摘　　要：为了解决大麻二酚酸(CBDA)传统提取工艺效率低、生长期和光质调控对其含量影响不明确等问题,试验遵循高效提取、精准调控理念,采用响应面法优化CBDA提取工艺,并考察光质调控汉麻11号不同生长期CBDA含量变化。根据单因素试验并结合响应面法考察液料比、提取温度、提取时间和超声波功率对CBDA含量的影响,确定最佳提取工艺并对理论结果进行验证。结果表明,CBDA最佳提取工艺为液料比50∶1(mL/g),提取温度33℃,提取时间32 min,超声波功率135 W,该条件下CBDA百分含量为3.885%,与验证结果(3.91±0.07)%基本一致,相比于以往文献报道方法提升了9.83%。光质调控研究表明,红色:绿色:蓝色=79.88%∶4.01%∶16.11%的光质条件下,初花期CBDA成分含量最高。该研究为CBDA的光质调控和最佳采收期提供了技术依据,有望提高CBDA的应用价值。This study aimed to solve the problems of the inefficiency of traditional cannabidiolic acid(CBDA)extraction and the unknow effect of different growth periods and light regulations on CBDA content.CBDA extraction was optimized through response surface methodology.CBDA content and changes of Hanma No.11 were analyzed under different light regulations and growth periods.The effects of liquidsolid ratio,extraction temperature,extraction time,and ultrasonic power on CBDA content were exam24 ined by single factor experiment and response surface methodology.The optimized extraction process was determined and the theoretical results were validated.The optimized extraction parameters for CBDA were:a liquid-to-material ratio of 50∶1(mL/g),an extraction temperature of 33°C,an extraction time of 32 min and an ultrasonic power of 135 W.CBDA content was 3.885% under these conditions,which was consistent with the experimental verification result of(3.91±0.07)%.Compared with the previous literature reporting method,it had been improved of 9.83%.The study of light regulations showed that CBDA content at the initial flowering stage was the highest when the light conditions were Red∶Green∶Blue=79.88%∶4.01%∶16.11%.The results of this study provide a theoretical basis for the light regulations and optimum harvest period of CBDA,and is expected to increase the application value of CBDA.

关 键 词：工业大麻 大麻二酚酸 提取工艺 光质调控 不同生长期 最佳采收期 

分 类 号：S563.3[农业科学—作物学] R284.2[医药卫生—中药学]