基于多指标含量测定及化学计量学的黔产黑龙骨茎叶质量评价  

Quality Evaluation of Periploca forrestii stem-leaf in Guizhou base on multiindex content determination combined with chemometrics

作　　者：周玲利 刘育辰 彭乐强 穆开朗 冉飞 刘刚 ZHOU Lingli;LIU Yuchen;PENG Leqiang;MU Kailang;RAN Fei;LIU Gang(Guizhou University of Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学,贵州贵阳550025

出　　处：《药物评价研究》2025年第2期455-461,共7页Drug Evaluation Research

基　　金：贵州省科技计划项目[黔科合基础-ZK(2022)一般475号];贵州省教育厅滚动支持省属高校科研平台团队项目[黔教技(2022)024号]。

摘　　要：目的建立黑龙骨Periploca forrestii茎叶(PFSL)中7个成分的UPLC测定方法,并对结果进行化学计量学分析。方法采用Agilent Eclipse Plus C18色谱柱(100 mm×2.1 mm,1.8μm),以0.1%磷酸水-乙腈为流动相梯度洗脱,体积流量0.2 mL·min^(-1),进样量0.8μL,柱温30℃;采用切换波长法测定15批PFSL中新绿原酸、绿原酸、咖啡酸、隐绿原酸、异槲皮苷、紫云英苷及异绿原酸C的含量,结合聚类分析(HCA)、主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLSDA)对含量测定结果进行综合评价。结果新绿原酸、绿原酸、咖啡酸、隐绿原酸、异槲皮苷、紫云英苷及异绿原酸C在0.03040~1.21600、0.01260~1.00800、0.00124~0.02480、0.01520~0.49600、0.01120~0.17920、0.00784~0.11760、0.00364~0.13600μg·mL^(-1)线性关系良好(R2>0.9990),平均加样回收率分别为96.01%、98.93%、98.52%、98.94%、98.06%、96.22%、98.20%,RSD分别为2.29%、3.13%、3.22%、2.71%、1.92%、2.86%、2.38%;质量分数分别为1.4131~12.2571、0.9121~10.0906、0.0424~0.3148、0.9266~8.5379、0.3506~3.3370、0.1764~0.6100、0.2531~1.2576 mg·g^(-1)。化学计量学分析结果显示,15批PFSL样品聚为3类,隐绿原酸、咖啡酸、紫云英苷、新绿原酸、绿原酸、异槲皮苷为15批样品中的主要差异成分。结论建立的UPLC含量测定方法稳定,结合化学计量学方法可为PFSL质量评价提供参考。Objective To establish an UPLC content determination method of seven components in Periploca forrestii stem-leaf(PFSL),and analyze the content determination results by chemometrics method.Methods The analysis was carried out on Agilent Eclipse Plus C18 column(100 mm×2.1 mm,1.8mm).The mobile phase was 0.1%phosphoric acid-acetonitrile with gradient elution.The volume flow was 0.2 mL·min^(−1),the injection volume was 0.8μL,and the column temperature was 30℃.Measuring the content of neochlorogenic acid,chlorogenic acid,caffeic acid,cryptochlorogenic acid,isoquercitrin,astragalin and isochlorogenic acid C in 15 batches of PFSL by wavelength switching method.The content determination results were comprehensively analyzed using hierarchical cluster analysis(HCA),principal component analysis(PCA),and orthogonal partial least squares-discriminant analysis(OPLS-DA).Results Neochlorogenic acid,chlorogenic acid,caffeic acid,cryptochlorogenic acid,isoquercitrin,astragalin and isochlorogenic acid C showed good linear relationship(R2>0.9990)within the ranges of 0.03040—1.21600,0.01260—1.00800,0.00124—0.02480,0.01520—0.49600,0.01120—0.17920,0.00784—0.11760,0.00364—0.13600mg·mL^(-1),whose average recoveries were 96.01%,98.93%,98.52%,98.94%,98.06%,96.22%and 98.20%,with RSD values of 2.29%,3.13%,3.22%,2.71%,1.92%,2.86%,2.38%,respectively.Their mass fractions were 1.4131—12.2571,0.9121—10.0906,0.0424—0.3148,0.9266—8.5379,0.3506—3.3370,0.1764—0.6100 and 0.2531—1.2576 mg·g^(-1).The chemometrics analysis results showed that 15 batches of PFSL could be clustered into three categories,cryptochlorogenic acid,caffeic acid,astragalin,neochlorogenic acid,chlorogenic acid and isoquercitrin were the main differential components in 15 batches of samples.Conclusion The established method for the determination of multicomponent content in PFSL is stable,which can provide reference for the quality evaluation of PFSL combined with chemometrics method.

关 键 词：黑龙骨茎叶 含量测定 化学识别模式 质量评价 新绿原酸 绿原酸 咖啡酸 隐绿原酸 异槲皮苷 紫云英苷 异绿原酸C 

分 类 号：R284.1[医药卫生—中药学]