MALDI-TOF MS对非O1/O139群霍乱弧菌的鉴定及聚类分析  

Identification and cluster analysis of non-O1/O139 Vibrio cholerae by MALDI-TOF MS

作　　者：徐茂锁 张慧 周聪 申春梅[2] 林勇 XU Maosuo;ZHANG Hui;ZHOU Cong;SHEN Chunmei;LIN Yong(Department of Laboratory Medicine,Shanghai Fifth People′s Hospital,Fudan University,Shanghai 200240,China;Department of Nosocomial Infection Prevention and Control,Shanghai Fifth People′s Hospital,Fudan University,Shanghai 200240,China)

机构地区：[1]复旦大学附属上海市第五人民医院,检验科,上海200240 [2]复旦大学附属上海市第五人民医院,院感科,上海200240

出　　处：《临床检验杂志》2025年第3期161-166,共6页Chinese Journal of Clinical Laboratory Science

基　　金：上海市闵行区医学特色专科建设项目(2025MWTZB01)。

摘　　要：目的 使用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)对非O1/O139群霍乱弧菌(NOVC)进行鉴定和聚类分析,评估MALDI-TOF MS作为NOVC鉴定和聚类方法的可行性。方法 采用MALDI-TOF MS搭载V5数据库、V12数据库、高致病性菌种数据库和V5数据库联合自建预测图谱对NOVC进行鉴定,采用主成分分析(PCA)和核心图谱(MSP)聚类对NOVC进行分析。结果 MALDI-TOF MS搭载V5数据库或V12数据库均将NOVC错误鉴定为易北河弧菌,而高致病性菌种数据库或V5数据库联合自建预测图谱可对NOVC正确鉴定;MALDI-TOF MS PCA聚类可将NOVC与其他菌种鉴别,并对NOVC进行种内细化区分,展示NOVC间距离关系;部分NOVC预测图谱与建库参考菌株极度相似,MSP聚类对NOVC种间差异无法区分。结论 MALDI-TOF MS对NOVC的鉴定能力受其数据库限制,高致病性菌种数据库或辅以自建预测图谱可准确鉴定NOVC;PCA聚类对NOVC种间和种内鉴别以及同源性分析具有一定的参考意义。Objective To identify and cluster non-O1/O139 Vibrio cholerae(NOVC)using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS),and evaluate the feasibility of MALDI-TOF MS as a method for the identification and clustering of NOVC.Methods The NOVC was identified by the MALDI-TOF MS equipped with V5 database,V12 database,highly pathogenic bacteria database,and V5 database combined with self-built spectrum projection and analyzed by the principal component analysis(PCA)and main spectrum projection(MSP)clustering.Results The NOVC was incorrectly identified as Vibrio albensis by the MALDI-TOF MS equipped with V5 database or V12 database,while the MALDI-TOF MS equipped with highly pathogenic bacteria database or V5 database combined with self-built spectrum projection could correctly identify NOVC.The PCA clustering of MALDI-TOF MS could distinguish NOVC from other bacterial strains and refine the differentiation of NOVC species to show the distance relationship between NOVC species.Some spectrum projections of NOVC were extremely similar to the reference strains used to establish the database,and MSP clustering could not distinguish the differences between NOVC species.Conclusion The identification ability of MALDI-TOF MS for NOVC is limited by its database.The MALDI-TOF MS equipped with highly pathogenic bacteria database or V5 database combined with the self-built spectrum projection can accurately identify NOVC.The PCA clustering of MALDI-TOF MS has certain reference significance for the intra-and inter-species identification and homology analysis of NOVC.

关 键 词：基质辅助激光解吸电离飞行时间质谱 非O1/O139群霍乱弧菌 主成分分析 

分 类 号：R446[医药卫生—诊断学] R372[医药卫生—临床医学]