卵巢癌患者外周血髓源性抑制细胞亚群比例及其临床意义  

Proportions and clinical significance of myeloid-derived suppressor cell subsets in the peripheral blood of ovarian cancer patients

作　　者：茅晔鹏 王婷 蔡针针 刘志洁 张月露 陶子琦 周凌飞 严丽娜 王芳[1,2] MAO Yepeng;WANG Ting;CAI Zhenzhen;LIU Zhijie;ZHANG Yuelu;TAO Ziqi;ZHOU Lingfei;YAN Lina;WANG Fang(Department of Laboratory Medicine,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,Jiangsu;Branch of National Clinical Research Center for Laboratory Medicine,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,Jiangsu;Department of Gynaecology,Nanjing Maternity and Child Health Care Hospital,Nanjing 210004,Jiangsu,China)

机构地区：[1]南京医科大学第一附属医院检验学部,南京210029 [2]南京医科大学第一附属医院国家医学检验临床医学研究中心分中心,南京210029 [3]南京市妇幼保健院妇科,南京210004

出　　处：《临床检验杂志》2025年第3期179-184,共6页Chinese Journal of Clinical Laboratory Science

基　　金：南京医科大学第一附属医院青年基金培育计划(PY2022032);江苏省医学会检验医学科研专项资金项目(ZDXK202239)。

摘　　要：目的 探究卵巢癌(OC)患者外周血中髓源性抑制细胞(MDSCs)及其亚群多形核髓源性抑制细胞(PMN-MDSCs)、早期髓源性抑制细胞(e-MDSCs)、单核髓源性抑制细胞(M-MDSCs)及凝集素型氧化低密度脂蛋白受体1(LOX-1)阳性PMN-MDSCs的比例,并分析其与患者临床病理参数的关系。方法 采用流式细胞术检测38例OC患者(OC组)和46例体检健康者(健康人对照组)外周血中MDSCs及其各亚群比例;利用ELISA法检测各组血清IL-10、TGF-β水平;进一步根据OC组LOX-1^(+)PMN-MDSCs在MDSCs比例的中位数将其分为LOX-1高表达组和低表达组。结果 OC组外周血单个核细胞(PBMC)中MDSC比例、PMN-MDSCs比例及LOX-1^(+)PMN-MDSCs比例均显著高于健康人对照组(U=492,P<0.001;t=8.741,P<0.0001;U=223,P<0.0001);OC组M-MDSCs比例及e-MDSCs比例均显著低于健康人对照组(t=4.366,P<0.0001;t=6.927,P<0.0001)。OC患者淋巴结转移组LOX-1^(+)PMN-MDSCs比例显著高于未转移组(t=2.249,P<0.05)。OC组血清IL-10、TGF-β水平显著高于健康人对照组(P<0.05);LOX-1高表达组血清TGF-β水平显著高于低表达组(t=2.302,P<0.05)。结论 OC患者外周血中LOX-1^(+)PMN-MDSCs比例显著升高,并与淋巴结转移密切相关。Objective To investigate the proportions of myeloid-derived suppressor cells(MDSCs)and their subsets,including polymorphonuclear MDSCs(PMN-MDSCs),early-stage MDSCs(e-MDSCs),monocytic MDSCs(M-MDSCs),and lectin-like oxidized low-density lipoprotein receptor-1(LOX-1)positive PMN-MDSCs,in the peripheral blood of ovarian cancer(OC)patients and analyze their correlations with clinicopathological parameters of the patients.Methods The proportions of MDSCs and their subsets in the peripheral blood of 38 OC patients(OC group)and 46 healthy individuals(healthy control group)were detected by flow cytometry.The levels of serum IL-10 and TGF-βwere detected using ELISA.The OC group was further divided into LOX-1 high and low expression subgroups based on the median proportion of LOX-1^(+)PMN-MDSCs in MDSCs.Results The proportions of MDSCs,PMN-MDSCs,and LOX-1^(+)PMN-MDSCs in the peripheral blood mononuclear cells(PBMCs)of the OC group were significantly higher than those in the healthy control group(U=492,P<0.001;t=8.741,P<0.0001;U=223,P<0.0001).The proportions of M-MDSCs and e-MDSCs in the OC group were significantly lower than those in the healthy control group(t=4.366,P<0.0001;t=6.927,P<0.0001).The proportion of LOX-1^(+)PMN-MDSCs in the lymph node metastasis group of OC patients was significantly higher than that in the non-metastasis group(t=2.249,P<0.05).The levels of serum IL-10 and TGF-βin the OC group were significantly higher than those in the healthy control group(P<0.05).In addition,the level of serum TGF-βin the LOX-1 high expression group was significantly higher than that in the LOX-1 low expression group(t=2.302,P<0.05).Conclusion The proportion of LOX-1^(+)PMN-MDSCs in the peripheral blood of OC patients is significantly increased and closely related to lymph node metastasis.

关 键 词：卵巢癌 髓源性抑制细胞 凝集素型氧化低密度脂蛋白受体-1 

分 类 号：R446.6[医药卫生—诊断学] R392.11[医药卫生—临床医学]