基于Cytb基因的牦牛肉种来源鉴定与应用  

Identification of Yak Breed Origin Based on the Cytb Gene and Application

作　　者：何建文[1,2] 党鹏举 金丽娜 韩建林[2] HE Jianwen;DANG Pengju;JIN Li'na;HAN Jianlin(Gansu Agricultural Vocational and Technical College,Lanzhou Gansu 730020,China;Gansu Key Laboratory of Herbivorous Animal Biotechnology,Gansu Agricultural University,Lanzhou Gansu 730070,China)

机构地区：[1]甘肃农业职业技术学院,甘肃兰州730020 [2]甘肃农业大学草食动物生物技术重点实验室,甘肃兰州730070

出　　处：《现代畜牧科技》2025年第3期71-74,共4页Modern Animal Husbandry Science & Technology

基　　金：CAAS-ILRI联合研究项目:动物遗传资源的分子评价(2008-CI-03);甘肃省教育科技创新基金项目(2022B-360)。

摘　　要：为建立牦牛、黄牛和水牛肉在基因水平快速、准确的鉴定方法,用于牦牛肉及制品的肉种来源鉴别研究。以牦牛、黄牛、水牛肉为研究对象,选用Cytb基因的一段保守区域对牦牛、黄牛和水牛序列进行限制性内切酶位点检测分析,发现3个特异性酶切位点可用于牦牛肉及制品的肉种来源的鉴别。运用该方法对不同品牌的牦牛肉产品进行检测,再通过测序验证酶切结果的正确性及其黄牛肉、水牛肉假冒牦牛肉制品的参杂程度。结果显示,3个特异性酶切位点可用于混合牛肉产品的鉴别,牦牛肉制品的掺假现象较为严重。In order to establish a rapid and accurate genetic identification method for yak,yellow cattle,and buffalo at the genetic level,for the source tracking and species identification of yak meat products.Using yak,yellow cattle,and buffalo as research materials,a pair of universal primers were used to amplify a conserved region of the Cytb gene in bovine mitochondria.PCR RFLP detection revealed three specific cleavage sites,which can be used for identifying the origin of mixed beef products.Using this method to detect yak meat products from different brands,and then verifying the accuracy of enzyme digestion results and the degree of contamination of counterfeit yak meat products from ordinary beef and water buffalo through sequencing.The results showed that three specific enzyme cleavage sites can be used for the identification of mixed beef products,and the phenomenon of using yellow cattle and water buffalo to impersonate yak meat products is more serious in the beef product market.

关 键 词：牦牛 PCR-RFLP CYTB基因 物种鉴定 

分 类 号：S823.85[农业科学—畜牧学]