HPLC法测定金花平喘散中盐酸麻黄碱、盐酸伪麻黄碱及苦杏仁苷的含量  

Determination of Ephedrine Hydrochloride,Pseudoephedrine Hydrochloride,and Amygdalin in Jinhua Pingchuan Powder by HPLC

作　　者：蔡文金 陈晓林 周炜 罗成江 周芷锦 沈红霞 陈勇 CAI Wen-jin;CHEN Xiao-lin;ZHOU Wei;LUO Chen-Jiang;ZHOU Zhi-Jin;SHEN Hong-Xia;CHEN Yong(Zhejiang Provincial Center for Animal Disease Prevention and Control,Hangzhou 311119,China)

机构地区：[1]浙江省动物疫病预防控制中心,杭州311119

出　　处：《中国兽药杂志》2025年第3期19-24,共6页Chinese Journal of Veterinary Drug

基　　金：浙江省重点研发项目(2024C02004)。

摘　　要：为了完善金花平喘散的质量控制方法,采用高效液相色谱法对金花平喘散中麻黄药材的有效成分盐酸麻黄碱、盐酸伪麻黄碱及苦杏仁中的有效成分苦杏仁苷的含量测定进行研究。以Waters X Bridge C18(4.6 mm×250 mm,5μm)为色谱柱,乙腈-0.2%磷酸溶液(梯度洗脱),流速1.0 mL/min,检测波长为210 nm,柱温30℃,进样量10μL。结果为盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷进样浓度分别在1.114~111.4μg/mL(r=0.99984)、1.111~111.089μg/mL(r=0.99981)、3.083~154.174μg/mL(r=0.99998)范围内,峰面积与含量呈良好线性关系。样品的平均回收率(n=6)分别为94.14%、98.42%、97.70%,RSD分别为1.93%、1.12%、0.66%。该方法简单准确度高,可用于金花平喘散中有效组分的含量测定,为控制其产品质量提供依据。In order to improve the quality control method of Jinhua Pianchuan powder,the content of effective components,ephedrine hydrochloride,pseudoephedrine hydrochloride and amygdalin in the herb of ephedra used in Jinhua Pianchuan powder were determined by high-performance liquid chromatography.Waters X Bridge C18(4.6 mm×250 mm,5μm)was used as the column,with a gradient elution of acetonitrile-0.2%phosphoric acid solution at a flow rate of 1.0 mL/min,a detection wavelength of 210 nm,and a column temperature of 30℃.The injection volume was 10μL.The results showed that the injection concentrations of ephedrine hydrochloride,pseudoephedrine hydrochloride and amygdalin were in the range of 1.114~111.4μg/mL(r=0.99984),1.111~111.089μg/mL(r=0.99981)and 3.083~154.174μg/mL(r=0.99998).There is a good linear relationship between peak area and content.The average recovery rates(n=6)were 94.14%,98.42%,and 97.70%,with RSD of 1.93%,1.12%,and 0.66%.The method is simple and accurate,and can be used for the determination of effective components in Jinhua Pingchuan powder,which provides a basis for the control of product quality.

关 键 词：高效液相色谱法 金花平喘散 盐酸麻黄碱 盐酸伪麻黄碱 苦杏仁苷 

分 类 号：S859.84[农业科学—临床兽医学]