ARTP诱变及高通量筛选选育米尔贝霉素高产菌株  

Breeding of Milbemycin High-yield Strain by ARTP Mutagenesis and High-throughput Screening

作　　者：张莉[1] 米贯东[1] 戴梦 谢新宇[1] 安兰兰 马婕 任风芝[1] ZHANG Li;MI Guan-dong;DAI Meng;XIE Xin-yu;AN Lan-lan;MA Jie;REN Feng-zhi(National Engineering Research Center of Microbial Medicine,Hebei Industry Microbial Metabolic Technology Innovation Centre,New Drug Research&Development Company of NCPC,Shijiazhuang 052165,China)

机构地区：[1]微生物药物国家工程研究中心,河北省工业微生物代谢技术创新中心,华北制药集团新药研究开发有限责任公司,石家庄052165

出　　处：《中国兽药杂志》2025年第3期25-33,共9页Chinese Journal of Veterinary Drug

基　　金：石家庄市后补助奖励专项资金项目(247790349H)。

摘　　要：为选育米尔贝霉素(milbemycin)优良菌株,提高发酵产量,以吸水链霉菌(Streptomyces hygroscopicus)MB230906作为出发菌株,通过常压室温等离子体(ARTP),结合自身代谢产物抗性,以及高通量筛选技术,获得了1株遗传性状稳定的高产突变菌株AM2405-228,50 L罐发酵产量平均达到3494μg/mL,较出发菌株提升28.31%,A3∶A4达到了1∶3.98。结果表明,运用ARTP诱变结合抗性和高通量筛选,可以高效、快速获得发酵产量显著提高的米尔贝霉素突变株,为进一步推进米尔贝霉素的工业化生产奠定坚实的研究基础。To breed an excellent strain of milbemycin and improve the fermentation yield,the Streptomyces hygroscopicus MB230906 was used as the starting strain.By employing the atmospheric pressure room temperature plasma(ARTP)combined with self-metabolite resistance and high-throughput screening technology,a high-yield mutant strain AM2405-228 with stable heredity was obtained.The average fermentation yield in a 50 L tank reached 3494μg/mL,which was 28.31%higher than that of the original strain,and the ratio of A3∶A4 reached 1∶3.98.The results demenstrated that ARTP mutagenesis combined with resistance and high-throughput screening could efficiently and rapidly obtain milbemycin mutant strains with significantly increased fermentation yields,which laid a solid research foundation for further promoting industrial production of milbemycin.

关 键 词：米尔贝霉素 吸水链霉菌 ARTP 高通量筛选 

分 类 号：S859.796[农业科学—临床兽医学]