靶向二代测序技术早期诊断鹦鹉热衣原体重症肺炎1例并文献复习  

Early diagnosis of chlamydia psittaci severe pneumonia by targeted next-generationsequencing technology:a case report and literature review

作　　者：王栋 李炼[1] 颜新生 WANG Dong;LI Lian;YAN Xinsheng(Department of Medical Laboratory,Wuhan Asia General Hospital/Wuhan Asia General Hospital Affiliated to Wuhan University of Science and Technology,Wuhan,Hubei 430056,China)

机构地区：[1]武汉亚心总医院/武汉科技大学附属武汉亚心总医院医学检验科,湖北武汉430056

出　　处：《现代医药卫生》2025年第3期653-656,共4页Journal of Modern Medicine & Health

摘　　要：2023年2月该院呼吸与危重症医学科收治1例运用靶向二代测序(tNGS)技术确诊的鹦鹉热衣原体重症肺炎患者,其因畏寒、发热伴头痛7 d伴胸闷、喘气2 d入院,初步诊断考虑重症肺炎、急性呼吸衰竭。对患者进行全面的实验室和影像学检查后,重症肺炎病因仍未查明。运用tNGS技术对患者肺泡灌洗液样本检出鹦鹉热衣原体(序列数757),从入院至确诊时间仅3 d。确定病原体后及时调整抗菌药物,采用莫西沙星治疗获得良好疗效。鹦鹉热衣原体感染可导致人类重症肺炎及急性呼吸衰竭,因其临床表现无特异性及传统病原学检测方法的局限性,导致早期诊断具有挑战性和治疗不及时。tNGS技术作为一种快速、准确、经济的病原微生物诊断方法,对早期诊断感染病原体不明的重症肺炎具有重要价值。In February 2023,a patient with severe chlamydia psittaci pneumonia diagnosed by targeted next-generation sequencing(tNGS)was admitted to the Department of Respiratory and Critical Care Medicine of the hospital.He was admitted to the hospital due to chills,fever,headache for seven days,chest tightness and wheezing for two days.The initial diagnosis was considered severe pneumonia and acute respiratory failure.The cause of severe pneumonia has not been identified after comprehensive laboratory and imaging examinations.The tNGS technique was used to detect Chlamydia psittaci(757 sequences)in bronchoalveolar lavage fluid samples of patients,and the time from admission to diagnosis was only three days.After the pathogen was determined,the antibacterial drugs were adjusted in time,and moxifloxacin was used to obtain good curative effect.Chlamydia psittaci infection can lead to severe pneumonia and acute respiratory failure in humans.Due to the non-specific clinical manifestations and the limitations of traditional etiological detection methods,early diagnosis is challenging and treatment is not timely.As a rapid,accurate and economical diagnostic method for pathogenic microorganisms,tNGS technology is of great value in the early diagnosis of severe pneumonia with unknown pathogens.

关 键 词：鹦鹉热衣原体 危重病 肺炎 靶向二代测序 早期诊断 病例报告 

分 类 号：R563.1[医药卫生—呼吸系统] R374.2[医药卫生—内科学]