Association analysis of an anti-obesity mechanism and key ripened Pu-erh tea bioactive components by mimicking human general tea drinking  

作　　者：Junyu Liu Zhengyang Song Haihong Chen Wen Zeng Guirong Han Wei Li Bing Xu Yuan Lu Canyang Zhang Zhenglian Xue Bin Lü Chong Zhang Song Yang Yi Wang Xinhui Xing 

机构地区：[1]MOE Key Laboratory for Industrial Biocatalysis,Centre for Synthetic and Systems Biology,Department of Chemical Engineering,Tsinghua University,Beijing 100084,China [2]Shenzhen International Graduate School,Tsinghua University,Shenzhen 518055,China [3]Institute of Biomedical Health Technology and Engineering,Shenzhen Bay Laboratory,Shenzhen 518055,China [4]Anhui University of Technology,Maanshan 243002,China [5]Yunnan Shujian Tea Co.,Ltd.,Kunming 650000,China [6]Shandong Province Key Laboratory of Applied Mycology,Qingdao International Center on Microbes Utilizing Biogas,School of Life Sciences,Qingdao Agricultural University,Qingdao 266109,China

出　　处：《Food Science and Human Wellness》2025年第2期450-468,共19页食品科学与人类健康(英文)

基　　金：The financial support received from the Shenzhen Science and Technology Innovation Commission(KCXFZ20201221173207022,WDZC20200821141349001);Shenzhen Bay Laboratory Startup Fund(21310041,S234602003)。

摘　　要：Pu-erh tea,a traditional Chinese beverage,performs an anti-obesity function,but the correlation between its components and efficacy remains unknown.Here,we screened two Pu-erh teas with significant anti-obesity efficacies from 11 teas.In vitro experiments revealed that lipid accumulation in L02 cells and lipid synthesis in 3T3-L1 cells were significantly better inhibited by Tea-B than Tea-A.Further in vivo experiments using model mice revealed that the differences in chemical components generated two pathways in the anti-obesity efficacy and mechanism of Pu-erh teas.Tea-A changes the histomorphology of brown adipose tissue(BAT)and increases the abundance of Coriobacteriaceae_UCG_002 and cyclic AMP in guts through high chemical contents of cyclopentasiloxane,decamethyl,tridecane and 1,2,3-trimethoxybenzene,eventually increasing BAT activation and fat browning gene expression;the high content of hexadecane and 1,2-dimethoxybenzene in Tea-B reduces white adipose tissue(WAT)accumulation and the process of fatty liver,increases the abundance of Odoribacter and sphinganine 1-phosphate,inhibits the expression of lipid synthesis and transport genes.These mechanistic findings on the association of the representative bioactive components in Pu-erh teas with the anti-obesity phenotypes,gut microbes,gut metabolite structure and anti-obesity pathways,which were obtained for the first time,provide foundations for developing functional Pu-erh tea.

关 键 词：ANTI-OBESITY Bioactive components Lipid metabolism Multiomics analysis Pu-erh tea 

分 类 号：R73[医药卫生—肿瘤]