Discovery and mechanism of anti-hypertensive effect of a novel tripeptide(SYP)from medicinal fungus Ganoderma lingzhi  

作　　者：Qiang Wu Feijun Luo Jia Huang Ping Huang Yongnan Liu Xiaoling Wang Gaoqiang Liu 

机构地区：[1]Hunan Provincial Key Laboratory of Forestry Biotechnology and International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology,Central South University of Forestry and Technology,Changsha 410004,China [2]Microbial Variety Creation Center,Laboratory of Yuelushan Seed Industry,Changsha 410004,China [3]College of Food and Chemical Engineering,Shaoyang University,Shaoyang 422000,China [4]Laboratory of Molecular Nutrition,National Engineering Laboratory for Rice and Byproducts,Central South University of Forestry and Technology,Changsha 410004,China

出　　处：《Food Science and Human Wellness》2025年第2期509-524,共16页食品科学与人类健康(英文)

基　　金：supported by the National Natural Science Foundation of China(32071673 and 32202573);the Program of Hunan Science and Technology Innovation Team(2021RC4063);the Natural Science Foundation of Hunan Province(2021JJ31151 and 2022JJ50028);the Key Scientific Research Project of Hunan Education Department(22A0538)。

摘　　要：Ganoderma lingzhi is a new species of the prize medicinal mushroom Ganoderma(Agaricomycetes).Using angiotensin I-converting enzyme(ACE)as a target,a tripeptide Ser-Tyr-Pro(SYP)was discovered with preponderant ACE inhibitory activity with an 50%inhibiting concentration(IC_(50))value of 62.50μg/mL attribute to the formed salt bridge and hydrogen bonds between SYP and ACE.SYP even maintained superior bioactivity after intestinal digestion,and exerted no cytotoxicity,but presented incomplete bioavailability in blood of spontaneous hypertensive rats(SHRs).Furthermore,it performed antihypertensive effect in vivo by inhibiting the influx of Ca^(2+)through activating endothelial NO synthase(e NOS)/NO/guanosine 3',5'-cyclic monophosphate(c GMP)pathway,accompanied by attenuating angiotensin II(Ang II)/NADPH oxidase(NOX)/reactive oxygen species(ROS)pathway.This work not only discoverers a novel pharmacological ingredient from medicinal mushroom G.lingzhi for hypertension therapy,but also provides an insight into molecular mechanism of the ACE inhibitory peptide(ACEIP)on lowering blood pressure.

关 键 词：Antihypertensive peptide Ganoderma lingzhi Gene microarray Molecular mechanism 

分 类 号：O62[理学—有机化学]