Nitric oxide mediates red light-induced perylenequinone production in Shiraia mycelium culture  

作　　者：Wen Juan Wang Xin Ping Li Wen Hao Shen Qun Yan Huang Rui Peng Cong Li Ping Zheng Jian Wen Wang 

机构地区：[1]College of Pharmaceutical Sciences,Soochow University,Suzhou 215123,China [2]Department of Horticultural Sciences,Soochow University,Suzhou 215123,China

出　　处：《Bioresources and Bioprocessing》2024年第1期11-27,共17页生物资源与生物加工(英文)

基　　金：supported by the National Natural Science Foundation of China(No.82073955 and 81773696);the Priority Academic Program Development of the Jiangsu Higher Education Institutes(PAPD).

摘　　要：Perylenequinones(PQs)from bambusicolous Shiraia fungi serve as excellent photosensitizers for photodynamic therapy.However,the lower yield of PQ production in mycelium cultures is an important bottleneck for their clinical application.Light has long been recognized as a pivotal regulatory signal for fungal secondary metabolite biosynthesis.In this study,we explored the role of nitric oxide(NO)in the growth and PQ biosynthesis in mycelium cultures of Shiraia sp.S9 exposed to red light.The continuous irradiation with red light(627 nm,200 lx)suppressed fungal conidiation,promoted hyphal branching,and elicited a notable increase in PQ accumulation.Red light exposure induced NO generation,peaking to 81.7μmol/g FW on day 8 of the culture,with the involvement of nitric oxide synthase(NOS)-or nitrate reductase(NR)-dependent pathways.The application of a NO donor sodium nitroprusside(SNP)restored conidiation of Shiraia sp.S9 under red light and stimulated PQ production,which was mitigated upon the introduction of NO scavenger carboxy-PTIO or soluble guanylate cyclase inhibitor NS-2028.These results showed that red light-induced NO,as a signaling molecule,was involved in the regulation of growth and PQ production in Shiraia sp.S9 through the NO-cGMP-PKG signaling pathway.While mycelial H2O2 content exhibited no significant alternations,a transient increase of intracellular Ca2+and extracellular ATP(eATP)content was detected upon exposure to red light.The generation of NO was found to be interdependent on cytosolic Ca2+and eATP concentration.These signal molecules cooperated synergistically to enhance membrane permeability and elevate the transcript levels of PQ biosynthetic genes in Shiraia sp.S9.Notably,the combined treatment of red light with 5μM SNP yielded a synergistic effect,resulting in a substantially higher level of hypocrellin A(HA,254 mg/L),about 3.0-fold over the dark control.Our findings provide valuable insights into the regulation of NO on fungal secondary metabolite biosynthesis and present a promising st

关 键 词：Shiraia Red light Nitric oxide Perylenequinone BIOSYNTHESIS ELICITATION 

分 类 号：S43[农业科学—农业昆虫与害虫防治]