Integrated characterization of filler tobacco leaves: HS-SPME-GC-MS, E-nose, and microbiome analysis across different origins  

作　　者：Mingzhu Zhang Dongfeng Guo Haiqing Wang Guanglong Wu Naihong Ding Yaqi Shi Jinlong Zhou Eryong Zhao Xingjiang Li 

机构地区：[1]Key Laboratory for Agricultural Products Processing,School of Food and Biological Engineering,Hefei University of Technology,Danxia Road 485#,Hefei City 230601,Anhui Province,China [2]China Tobacco Anhui Industrial Co.,Ltd.,Huangshan Road 606#,Hefei City 230088,Anhui Province,China

出　　处：《Bioresources and Bioprocessing》2024年第1期156-173,共18页生物资源与生物加工(英文)

基　　金：funded by the State-Owned Enterprises Cooperative External Program,Grant Number W2023JSKF0083.

摘　　要：This study delves into the aroma characteristics and microbial composition of filler tobacco leaves(FTLs)sourced from six distinct cigar-growing regions within Yunnan,China,following standardized fermentation.An integrated approach using gas chromatography-mass spectrometry(GC-MS),electronic nose(E-nose),and microbiome analysis was employed for comprehensive profiling.Results derived from Linear Discriminant Analysis(LDA)using E-nose data confirmed the presence of notable variability in flavor substance profiles among the FTLs from six regions.Additionally,GC-MS was used to discern disparities in volatile organic compound(VOC)distribution across FTLs from these regions,identifying 92,81,79,58,69,and 92 VOCs within each respective sample set.Significantly,24 VOCs emerged as pivotal determinants contributing to the heterogeneity of flavor profiles among FTLs from diverse origins,as indicated by Variable Importance for the Projection(VIP)analysis.Furthermore,distinctions in free amino acid content and chemical constituents were observed across FTLs.Of noteworthy significance,solanone,isophorone,durene,(-)-alpha-terpineol,and 2,3’-bipyridine exhibited the strongest correlations with microbiome data,with fungal microorganisms exerting a more pronounced influence on metabolites,as elucidated through two-way orthogonal partial least-squares(O2PLS)modeling.These findings provide a theoretical and technical basis for accurately evaluating the synchronization of FTLs in aromas and fermentation processes,and they will enhance the quality of fermented FTLs and foster the growth of the domestic cigar tobacco industry ultimately.

关 键 词：Filler tobacco leaves Volatile organic compounds Microbial community Chemical constituents Yunnan region 

分 类 号：O62[理学—有机化学]