Collagen type II solution extracted from supercritical carbon dioxide decellularized porcine cartilage: regenerative efficacy on post-traumatic osteoarthritis model  

作　　者：Srinivasan Periasamy Yun-Ju Chen Dur-Zong Hsu Dar-Jen Hsieh 

机构地区：[1]R&D Center,ACRO Biomedical Co.,Ltd,2nd.Floor,No.57,Luke 2nd.Rd.,Luzhu District,Kaohsiung City 82151,Taiwan,China [2]Department of Environmental and Occupational Health,College of Medicine,National Cheng Kung University,138 Sheng-Li Rd.,Tainan 70428,Taiwan,China

出　　处：《Bioresources and Bioprocessing》2024年第1期281-294,共14页生物资源与生物加工(英文)

摘　　要：Osteoarthritis(OA)of the knee is a common degenerative articular disorder and is one of the main causes of pain and functional disability.Cartilage damage is frequently linked to elevated osteoarthritis incidence.Supercritical carbon dioxide(scCO2)decellularized cartilage graft produced from the porcine cartilage is an ideal candidate for cartilage tissue engineering.In the present study,we derived collagen type II(Col II)solution from the scCO2 decellularized porcine cartilage graft(dPCG)and compared its efficacy with hyaluronic acid(HA)in the surgical medial meniscectomy(MNX)induced post-traumatic osteoarthritis(PTOA)model.Dose-dependent attenuation of the OA(12.3±0.8)progression was observed in the intra-articular administration of Col II solution(7.3±1.2)which significantly decreased the MNX-induced OA symptoms similar to HA.The pain of the OA group(37.4±2.7)was attenuated dose-dependently by Col II solution(45.9±4.1)similar to HA(43.1±3.5)as evaluated by a capacitance meter.Micro-CT depicted a dose-dependent attenuation of articular cartilage damage by the Col II solution similar to HA treatment.A significant(p<0.001)dose-dependent elevation in the bone volume was also observed in Col II solution-treated OA animals.The protective competence of Col II solution on articular cartilage damage is due to its significant(p<0.001)increase in the expression of type II collagen,aggrecan and SOX-9 similar to HA.To conclude,intra-articular adminis-tration of type II collagen solution and HA reestablished the injured cartilage and decreased osteoarthritis progression in the experimental PTOA model.

关 键 词：Type II collagen solution Supercritical carbon dioxide(sc CO_(2)) Decellularized porcine cartilage graft 

分 类 号：R28[医药卫生—中药学]