Nutrients,surfactants,and aeration in constructed wetlands affect bacterial persistence and metabolic activity during the remediation of crude oil-contaminated water  

作　　者：Amer Jamal Hashmat Muhammad Afzal Samina Iqbal Imran Amin Carlos Alberto Arias Hans Brix Imran Zafar Sania Riaz Rizwan ur Rehman Ahmad Mohammad Salamatullah Gezahign Fentahun Wondmie Mohammed Bourhia 

机构地区：[1]National Institute for Biotechnology and Genetic Engineering College,Pakistan Institute of Engineering and Applied Sciences(NIBGE-C,PIEAS),Faisalabad,Punjab 38000,Pakistan [2]Centre for Water Technology(WATEC),Aarhus University,Aarhus C 8000,Denmark [3]Department of Bioinformatics and Computational Biology,Virtual University Pakistan,Lahore,Pakistan [4]Faculty of Health and Life Sciences,Department of Bioinformatics and Biosciences,Capital Territory,Capital University of Science and Technology,Zone V,Kahuta Road,Islamabad,Pakistan [5]Department of Food Science&Nutrition,College of Food and Agricultural Sciences,King Saud University,11,P.O.Box 2460,Riyadh 11451,Saudi Arabia [6]Department of Biology,Bahir Dar University,P.O.Box 79,Bahir Dar,Ethiopia [7]Laboratory of Biotechnology and Natural Resources Valorization,Faculty of Sciences,Ibn Zohr University,Agadir 80060,Morocco [8]Department of Biology,Aarhus University,Aarhus,Denmark

出　　处：《Bioresources and Bioprocessing》2024年第1期568-575,共8页生物资源与生物加工(英文)

基　　金：the Researchers Supporting Project number(RSP2024R437);King Saud University,Riyadh,Saudi Arabia;The authors are thankful to the Higher Education Commission(HEC)for the grant of IRSIP Fellowship to the lead author for research at Aarhus University Denmark and ALP Pakistan Agriculture Research Council(PARC)grant No.NR-086.

摘　　要：The use of constructed wetlands(CWs)is one of the best options to treat wastewater.In CWs,microorganisms play a major role in the degradation of organic pollutants but the concentration of nutrients,surfactant,and aeration(NSA)in oil-contaminated water is one of the factors that affect the persistence and metabolic functioning of hydrocarbon-degrading microorganisms.In the present investigation,the influence of the addition of NSA on the persistence of the augmented bacteria,copy of(alkane hydroxylase gene)alkB gene,and its expression level in the water,soil,and plants of CWs were evaluated.The CWs mesocosms were developed by the vegetation of Typha latifolia and Cyperus laevigatus and inoculated with the bacterial consortium(Pseudomonas putida TYRI39,Acinetobacter junii TYRH47,Acinetobacter sp.CYRH17,Pseudomonas sp.CYSI27,and Pseudomonas sp.TYRH42).The mesocosms were provided with nutrients(20 mg l−1 N,2.6 mg l−1 P,and 16.4 mg l−1 K)in liquid form,surfactant Tween-20(0.2%,v/v)in liquid form,and aeration(≥7.0±1 mg l−1 DO)using aeration pump.The addition of NSA in CWs enhanced the persistence and metabolic functioning of the inoculated bacteria in the water,rhizospheric soil,and plants.The maximum hydrocarbon removal(97%)was observed in the water treated by CWs having C.laevigatus,bacteria,and NSA,and it is correlated with the copy numbers of alkB and its expression level.The application of NSA in CWs not only improved bacterial persistence and catabolic gene expression but also increased plant development and hydrocarbon removal.

关 键 词：Crude oil Constructed wetlands Bacterial persistence Metabolic activity Gene abundance and expression 

分 类 号：TV1[水利工程]