Co-production of 1,3-propanediol and phage phiKpS2 from the glycerol fermentation by Klebsiella pneumoniae  

作　　者：Suyang Duan Zhirong Zhang Xiaoli Wang Yaqin Sun Yuesheng Dong Lina Ren Lili Geng Zhilong Xiu 

机构地区：[1]School of Bioengineering,Dalian University of Technology,Linggong Road 2,Dalian 116024,P.R.China [2]Department of Respiratory,Affiliated Dalian Municipal Central Hospital of Dalian University of Technology,Dalian 116033,Liaoning,P.R.China

出　　处：《Bioresources and Bioprocessing》2024年第1期610-620,共11页生物资源与生物加工(英文)

基　　金：Funding was provided by the National Natural Science Foundation of China(Grant No.22078042).

摘　　要：As an alternative to antibiotics in response to antimicrobial-resistant infections,bacteriophages(phages)are garnering renewed interest in recent years.However,the massive preparation of phage is restricted using traditional pathogens as host cells,which incurs additional costs and contamination.In this study,an opportunistic pathogen,Klebsiella pneumoniae used to convert glycerol to 1,3-propanediol(1,3-PDO),was reused to prepare phage after fermentation.The phage infection showed that the fed-batch fermentation broth containing 71.6 g/L 1,3-PDO can be directly used for preparation of phage with a titer of 1×108 pfu/mL.Then,the two-step salting-out extraction was adopted to remove most impurities,e.g.acetic acid(93.5%),ethanol(91.5%)and cells(99.4%)at the first step,and obtain 1,3-PDO(56.6%)in the top phase as well as phage(97.4%)in the middle phase at the second step.This integrated process provides a cheap and environment-friendly manner for coproduction of 1,3-PDO and phage.

关 键 词：Klebsiella pneumonia 1 3-Propanediol BACTERIOPHAGE CO-PRODUCTION Salting-out extraction 

分 类 号：TQ9[化学工程]