Whole-cell one-pot biosynthesis of dodecanedioic acid from renewable linoleic acid  

作　　者：Yi-Ke Qi Jiang Pan Zhi-Jun Zhang Jian-He Xu 

机构地区：[1]State Key Laboratory of Bioreactor Engineering,School of Biotechnology,East China University of Science and Technology,130 Meilong Road,Shanghai 200237,China [2]College of Food Science and Biology,Hebei University of Science and Technology,26 Yuxiang Street,Shijiazhuang 050018,China

出　　处：《Bioresources and Bioprocessing》2024年第1期753-762,共10页生物资源与生物加工(英文)

基　　金：the financial supports from the National Key Research and Development Program of China(Nos.2019YFA0905000&2022YFC2105900);National Natural Science Foundation of China(Nos.32071475&21536004).

摘　　要：Background Dodecanedioic acid(DDA),a typical medium-chain dicarboxylic fatty acid with widespread applications,has a great synthetic value and a huge industrial market demand.Currently,a sustainable,eco-friendly and efficient process is desired for dodecanedioic acid production.Results Herein,a multi-enzymatic cascade was designed and constructed for the production of DDA from linoleic acid based on the lipoxygenase pathway in plants.The cascade is composed of lipoxygenase,hydroperoxide lyase,aldehyde dehydrogenase,and unidentified double-bond reductase in E.coli for the main cascade reactions,as well as NADH oxidase for cofactor recycling.The four component enzymes involved in the cascade were co-expressed in E.coli,together with the endogenous double-bond reductase of E.coli.After optimizing the reaction conditions of the rate-limiting step,43.8 g L−1 d−1 of DDA was obtained by a whole-cell one-pot process starting from renewable linoleic acid.Conclusions Through engineering of the reaction system and co-expressing the component enzymes,a sustainable and eco-friendly DDA biosynthesis route was set up in E.coli,which afforded the highest space time yield for DDA production among the current artificial multi-enzymatic routes derived from the LOX-pathway,and the productivity achieved here ranks the second highest among the current research progress in DDA biosynthesis.

关 键 词：Linoleic acid Dodecanedioic acid Escherichia coli Whole-cell biosynthesis Multi-enzymatic cascade 

分 类 号：O62[理学—有机化学]