Co-immobilization of amine dehydrogenase and glucose dehydrogenase for the biosynthesis of(S)-2-aminobutan-1-ol in continuous flow  

作　　者：Pengcheng Xie Jin Lan Jingshuan Zhou Zhun Hu Jiandong Cui Ge Qu Bo Yuan Zhoutong Sun 

机构地区：[1]College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China [2]Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China [3]Key Laboratory of Engineering Biology for Low-Carbon Manufacturing,Chinese Academy of Sciences,Tianjin Airport Economic Area,32 West 7th Avenue,Tianjin 300308,China [4]Institute of Industrial Catalysis,School of Chemical Engineering and Technology,Xi’an Jiaotong University,Xi’an Shaanxi 710049,China

出　　处：《Bioresources and Bioprocessing》2024年第1期946-956,共11页生物资源与生物加工(英文)

基　　金：supported by the National Key Research and Development Program of China(No.2021YFA0910400 and 2019YFA0905100);the National Natural Science Foundation of China(No.32171462);Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(No.TSBICIP-CXRC-040);the Natural Science Foundation of Tianjin(No.21JCJQJC00110).

摘　　要：Reductive amination by amine dehydrogenases is a green and sustainable process that produces only water as the by-product.In this study,a continuous flow process was designed utilizing a packed bed reactor filled with co-immobilized amine dehydrogenase wh84 and glucose dehydrogenase for the highly efficient biocatalytic synthesis of chiral amino alcohols.The immobilized amine dehydrogenase wh84 exhibited better thermo-,pH and solvent stability with high activity recovery.(S)-2-aminobutan-1-ol was produced in up to 99%conversion and 99%ee in the continuous flow processes,and the space-time yields were up to 124.5 g L-1 d-1.The continuous reactions were also extended to 48 h affording up to 91.8%average conversions.This study showcased the important potential to sustainable production of chiral amino alcohols in continuous flow processes.

关 键 词：Continuous flow Asymmetric reductive amination Amine dehydrogenases CO-IMMOBILIZATION Packed bed reactor 

分 类 号：O62[理学—有机化学]