Stirring the hydrogen and butanol production from Enset fiber via simultaneous saccharification and fermentation(SSF)process  

作　　者：Nebyat Seid Lea Wießner Habibu Aliyu Anke Neumann 

机构地区：[1]Electrobiotechnology,Institute of Process Engineering in Life Science 2,Karlsruhe Institute of Technology(KIT),76131 Karlsruhe,Germany [2]School of Chemical and Bio Engineering,Addis Ababa Institute of Technology,Addis Ababa University,P.O.B:1176,Addis Ababa,Ethiopia [3]Institute for Biological Interfaces 5,Karlsruhe Institute of Technology(KIT),76344 Karlsruhe,Germany

出　　处：《Bioresources and Bioprocessing》2024年第1期1269-1287,共19页生物资源与生物加工(英文)

基　　金：Open Access funding enabled and organized by Projekt DEAL;funded by the DAAD-EECBP Homegrown Ph.D.Scholarship Program 2019,and the APC was funded by the Karlsruhe Institute of Technology(KIT)Publication Fund.

摘　　要：Enset fiber is a promising feedstock for biofuel production with the potential to reduce carbon emissions and improve the sustainability of the energy system.This study aimed to maximize hydrogen and butanol production from Enset fiber through simultaneous saccharification and fermentation(SSF)process in bottles as well as in bioreactor.The SSF process in bottles resulted in a higher butanol concentration of 11.36 g/L with a yield of 0.23 g/g and a productivity of 0.16 g/(L h)at the optimal process parameters of 5%(w/v)substrate loading,16 FPU/g cellulase loading,and 100 rpm agitation speed from pretreated Enset fiber.Moreover,a comparable result to the bottle experiment was observed in the bioreactor with pH-uncontrolled SSF process,although with a decreased in butanol productivity to 0.095 g/(L h).However,using the pre-hydrolysis simultaneous saccharification and fermentation(PSSF)process in the bioreactor with a 7%(w/v)substrate loading led to the highest butanol concentration of 12.84 g/L with a productivity of 0.104 g/(L h).Furthermore,optimizing the SSF process parameters to favor hydrogen resulted in an increased hydrogen yield of 198.27 mL/g-Enset fiber at atmospheric pressure,an initial pH of 8.0,and 37°C.In general,stirring the SSF process to shift the product ratio to either hydrogen or butanol was possible by adjusting temperature and pressure.At 37°C and atmospheric pressure,the process resulted in an e-mol yield of 12%for hydrogen and 38%for butanol.Alternatively,at 30°C and 0.55 bar overpressure,the process achieved a yield of 6%e-mol of hydrogen and 48%e-mol of butanol.This is the first study to produce hydrogen and butanol from Enset fiber using the SSF process and contributes to the development of a circular bioeconomy.

关 键 词：HYDROGEN BUTANOL Enset fiber C.saccharoperbutylacetonicum SSF PSSF 

分 类 号：TG1[金属学及工艺—金属学]