Precise regulating the specific oxygen consumption rate to strengthen the CoQ10 biosynthesis by Rhodobater sphaeroides  

作　　者：Bo Li Yan Ge Jianguang Liang Zhichun Zhu Biqin Chen Dan Li Yingping Zhuang Zejian Wang 

机构地区：[1]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,130 Meilong Rd,P.O.box 329#,Shanghai 200237,China [2]College of Pharmaceutical and Life Sciences,Changzhou University,Changzhou 213164,China [3]Inner Mongolia Kingdomway Pharmaceutical Company,Hohhot 010000,China

出　　处：《Bioresources and Bioprocessing》2024年第1期1378-1387,共10页生物资源与生物加工(英文)

基　　金：supported by the financial support of research projects from the National Key R&D Program of China(No.2021YFC2101000);Shanghai Scientific and Technological Innovation Action Plans-Scientific Instrument Development,China(grant No.21142201300,22142201000);National Natural Science Foundation of China(No.32071471 and No.22078295);Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX21_2866).

摘　　要：Coenzyme Q10(CoQ10)is the most consumed dietary supplement and mainly biosynthesized by aerobic fermentation of Rhodobacter sphaeroides(R.sphaeroides).Oxygen supply was identified as a bottleneck for improving CoQ10 yield in R.sphaeroides.In this study,a precise regulation strategy based on dielectric spectroscopy(DS)was applied to further improve CoQ10 biosynthesis by R.sphaeroide.First,a quantitative response model among viable cells,cell morphology,and oxygen uptake rate(OUR)was established.DS could be used to detect viable R.sphaeroides cells,and the relationship among cell morphology,CoQ10 biosynthesis,and OUR was found to be significant.Based on this model,the online specific oxygen consumption rate(QO2)control strategy was successfully applied to the CoQ10 fermentation process.QO2 controlled at 0.07±0.01×10−7mmol/cell/h was most favorable for CoQ10 biosynthesis,resulting in a 28.3%increase in CoQ10 production.Based on the multi-parameters analysis and online QO2 control,a precise online nutrient feeding strategy was established using conductivity detected by DS.CoQ10 production was improved by 35%,reaching 3384 mg/L in 50 L bioreactors.This online control strategy would be effectively applied for improving industrial CoQ10 production,and the precise fermentation control strategy could also be applied to other fermentation process.

关 键 词：Coenzyme Q10 Specific oxygen consumption rate Rhodobater sphaeroides Dielectric spectroscopy Morphology 

分 类 号：TQ9[化学工程]