Optimized production of bacterioruberin from“Haloferax marinum”using one-factor-at-a-time and central composite design approaches  

作　　者：Eui-Sang Cho Chi Young Hwang Myung-Ji Seo 

机构地区：[1]BioTechnology Institute,University of Minnesota,St.Paul,MN 55108,USA [2]Department of Bioengineering and Nano-Bioengineering,Incheon National University,Incheon 22012,Republic of Korea [3]Division of Bioengineering,Incheon National University,Incheon 22012,Republic of Korea [4]Research Center for Bio Materials and Process Development,Incheon National University,Incheon 22012,Republic of Korea

出　　处：《Bioresources and Bioprocessing》2024年第1期1458-1470,共13页生物资源与生物加工(英文)

基　　金：supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(2022R1F1A1062699).

摘　　要：Haloarchaea represents a unique group of microorganisms that have adapted to thrive in high-salt environments.These microbes produce distinctive biomolecules,some of which exhibit extraordinary properties.One such biomolecule is bacterioruberin,a prominent red-pigmented C50 carotenoid commonly found in halophilic archaea,renowned for its antioxidant properties and potential as a functional resource.This study aimed to enhance the culture conditions for optimal production of C50 carotenoids,primarily bacterioruberin,using“Haloferax marinum”MBLA0078.The optimization process involved a combination of one-factor-at-a-time(OFAT)and statistical methodology.Under OFAT-optimized conditions,fed-batch fermentation,and response surface methodology(RSM)optimization,carotenoid production reached 0.954 mg/L,2.80 mg/L,and 2.16 mg/L,respectively,in a 7-L laboratory-scale fermenter.Notably,RSM-optimized conditions led to a 12-fold increase in productivity(0.72 mg/L/day)compared to the basal DBCM2 medium(0.06 mg/L/day).These findings suggest that strain MBLA0078 holds significant promise for commercial-scale production of bacterioruberin.

关 键 词：Bacterioruberin Haloferax marinum CAROTENOID Optimization 

分 类 号：F42[经济管理—产业经济]