沉积物对普通小球藻生长影响的模拟实验研究  

Simulated test study on effect of sediment on growth of Chlorella vulgaris

作　　者：何立乐 朱小雪 张振华[1,2] HE Lile;ZHU Xiaoxue;ZHANG Zhenhua(Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology of Chinese Academy of Science,Xining 810008,China;Qinghai Haibei National Field Research Station of Alpine Grassland Ecosystem,Northwest Institute of Plateau Biology of Chinese Academy of Sciences,Xining 810008,China;School of Biology Resources and Food Engineering,Qujing Normal University,Qujing 655011,China)

机构地区：[1]中国科学院西北高原生物研究所高原生物适应与进化重点实验室,青海西宁810008 [2]中国科学院西北高原生物研究所青海海北高寒草地生态系统国家野外科学观测研究站,青海西宁810008 [3]曲靖师范学院生物资源与食品工程学院,云南曲靖655011

出　　处：《人民长江》2025年第3期73-78,共6页Yangtze River

基　　金：云南省基础研究计划项目-青年项目(2019FD105);中科院-青海省三江源国家公园联合研究专项(LHZX-2021-01)。

摘　　要：为了揭示沉积物条件对普通小球藻生长的影响,采用纯石英砂模拟水体沉积物,用正交实验法分析了扰动速率、固液比和沉积物粒径等3种因素对普通小球藻生长的影响。结果表明:①扰动速率和固液比在0.05水平上对普通小球藻生物量均具有显著性影响,扰动速率和粒径在0.05水平上对普通小球藻特定生长率均具有显著性影响。②影响普通小球藻生物量达到最大的沉积物条件为100 r/min扰动速率、250 g/L固液比和10~20目粒径,影响普通小球藻生物量达到最小的沉积物条件为50 r/min扰动速率、125 g/L固液比和30~40目粒径。在一定的扰动速率下,粒径和固液比可能对特定生长率存在较强交互作用影响。③SEM显示较强的扰动速率能使普通小球藻外在形态椭圆化,并对生物量的增长产生影响。因此,研究认为可以试图通过改变水体扰动速率、固液比和沉积物粒径等沉积物条件实现对普通小球藻生长的宏观调控。In order to reveal the influence of water sediment conditions on growth of Chlorella vulgaris,pure quartz sand was used to simulate water sediments,and the orthogonal experimental method was used to analyze effects of disturbance rate,solid-liquid ratio,and sediment particle size on the growth of Chlorella vulgaris.The results showed that:①the disturbance rate and solid-liquid ratio had significant effects on the biomass of Chlorella vulgaris at a level of 0.05,and the disturbance rate and particle size had significant effects on the specific growth rate of Chlorella vulgaris at a level of 0.05.②The dominant conditions affecting the maximum biomass of Chlorella vulgaris were disturbance rate of 100 r/min,solid-liquid ratio of 250 g/L,particle size of 10~20 mesh.The dominant conditions affecting the minimum biomass of Chlorella vulgaris were disturbance rate of 50 r/min,solid-liquid ratio of 125 g/L,particle size of 30~40 mesh.Particle size and solid-liquid ratio may have a strong interaction effect on specific growth rate at a certain disturbance rate.③SEM showed that a strong disturbance rate could make the external morphology of Chlorella vulgaris elliptical,which would have an impact on the growth of biomass.Therefore,changing water sediment conditions such as disturbance rate,solid-liquid ratio,and sediment size is suggested for macro-control of Chlorella vulgaris growth.

关 键 词：普通小球藻 生物量 扰动速率 固液比 沉积物粒径 正交实验 

分 类 号：Q945[生物学—植物学]