降脂胶囊通过TLR4/MyD88/NF-κB信号通路抑制ApoE^(-/-)小鼠动脉粥样硬化炎症反应  

Jiangzhi capsule inhibits atherosclerotic inflammatory response in ApoE^(-/-)mice via TLR4/MyD88/NF-κB signaling pathway

作　　者：张曙光[1] 李宝同[1] 李鹤[1] 徐素娥 张少红 Zhang Shuguang;Li Baotong;Li He;Xu Su'e;Zhang Shaohong(Department of Cardiology,Huai'an TCM Hospital Affiliated to Nanjing University of Chinese Medicine,Huai'an,Jiangsu,223001,P.R.China;Department of Geriatrics,Huai'an First People's Hospital Affiliated to Nanjing Medical University,Huai'an,Jiangsu,223300,P.R.China)

机构地区：[1]南京中医药大学附属淮安中医院心血管病科,江苏淮安223001 [2]南京医科大学附属淮安第一医院老年医学科,江苏淮安223300

出　　处：《老年医学与保健》2025年第1期114-119,130,共7页Geriatrics & Health Care

基　　金：淮安市自然科学研究计划(联合专项)卫生健康类科研项目(HABL202254);江苏省中医药科技发展计划项目(MS2024099);淮安市科技计划项目(HAB202313);国家自然科学基金青年项目(82305149);江苏省老年健康科研项目(LKM2024045)。

摘　　要：目的观察降脂胶囊对ApoE^(-/-)小鼠动脉粥样硬化模型血脂、主动脉粥样硬化及TLR4/MyD88/NF-κB信号通路中关键蛋白及炎症因子的影响,探讨降脂胶囊治疗动脉粥样硬化的作用机制。方法将ApoE^(-/-)小鼠随机分为标准饮食(CON)和高脂饮食组(HFD)。HFD组持续12周制备动脉粥样硬化模型。然后将高脂饮食喂养小鼠进一步分为模型组,阿托伐他汀(4.11 mg·kg^(-1))组,低剂量降脂胶囊(0.62 g·kg^(-1))组,高剂量降脂胶囊(1.24 g·kg^(-1))组,给药12周。取主动脉进行HE染色,检测血脂TG、TC、HDL-c和LDL-c水平。采用Elisa及Western blot方法检测炎症因子TNF-α、IL-1β、ICAM-1和MCP-1水平,采用Western blot方法检测TLR4、MyD88、NF-κBp65蛋白表达水平。结果降脂胶囊降低ApoE^(-/-)小鼠AS模型血脂水平,降低炎症因子TNF-α、IL-1β、ICAM-1、MCP-1水平及TLR4、MyD88、NF-κBp65蛋白表达。结论降脂胶囊通过抑制TLR4/MyD88/NF-κB通路中关键蛋白及炎症因子的表达水平抑制AS的炎症反应。Objective To observe the effects of Jiangzhi capsule on blood lipid,aortic atherosclerosis(AS),key proteins in TLR4/MyD88/NF-κB signaling pathway and inflammatory factors in an ApoE^(-/-)mouse AS model,and explore its mechanism in the treatment of atherosclerosis.Methods ApoE^(-/-)mice were randomly divided into standard diet group(control group)and high-fat diet group(HFD group).The HFD group was fed with high-fat diet for 12 weeks to establish an AS model.Subsequently,the mice in the HFD group were further subdivided into model group,atorvastatin group(4.11 mg/kg),low-dose Jiangzhi capsule group(0.62 g/kg),and high-dose Jiangzhi capsule group(1.24 g/kg).The duration of administration was 12 weeks.Aortic tissues were collected for hematoxylin-eosin(HE)staining,and blood lipid levels(TG,TC,HDL-c,and LDL-c)were measured.Enzyme-linked immunosorbent assay(ELISA)and Western blot were used to measure the levels of inflammatory factors(TNF-α,IL-1β,ICAM-1,MCP-1),and Western blot was employed to measure protein expression levels of TLR4,MyD88 and NF-κBp65.Results Jiangzhi capsule significantly decreased the levels of blood lipid level,inflammatory factors(TNF-α,IL-1β,ICAM-1,MCP-1)and expression of TLR4,MyD88,and NF-κBp65 proteins in the AS model of ApoE^(-/-)mice.Conclusion Jiangzhi capsule can inhibit the inflammatory response of AS by inhibiting the expression levels of key proteins in TLR4/MyD88/NF-κB pathway and inflammatory factors.

关 键 词：降脂胶囊 动脉粥样硬化 TLR4/MyD88/NF-κB信号通路 炎症反应 

分 类 号：R28[医药卫生—中药学]