胚蛋给养核黄素对鸡骨骼肌发育的影响  

The Effect of Riboflavin Supplementation in Embryonic Eggs on the Development of Skeletal Muscle of Chickens

作　　者：李远方 张鸿源 李鸿泰 李智[1,2] 魏千然 王亚东[1,2] 李国喜 王丹丹[5] 刘翘铭 LI Yuanfang;ZHANG Hongyuan;LI Hongtai;LI Zhi;WEI Qianran;WANG Yadong;LI Guoxi;WANG Dandan;LIU Qiaoming(School of Medicine and Health,Harbin Institute of Technology,Harbin 150001,China;Zhengzhou Research Institute,Harbin Institute of Technology,Zhengzhou 450000,China;The Shennong Laboratory,Zhengzhou 450002,China;College of Animal Science and Technology,Henan Agricultural University,Zhengzhou 450046,China;Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]哈尔滨工业大学医学与健康学院,哈尔滨150001 [2]哈尔滨工业大学郑州研究院,郑州450000 [3]神农种业实验室,郑州450002 [4]河南农业大学动物科技学院,郑州450046 [5]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《畜牧兽医学报》2025年第3期1159-1169,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：鸡生长性状精准评价与高产优质核心种质创制(SN01-2022-05);重要农业动植物优异性状共性调控元件挖掘与基因资源设计(2023YFF1001100);中国博士后科学基金面上项目(GZC20233467)。

摘　　要：旨在研究核黄素(又称维生素B2,VB2)对鸡胚胎骨骼肌发育的影响,以促进其在胚胎给养中的应用。本研究利用CCK-8、EdU和免疫荧光等检测方法,鉴定添加不同浓度的VB2(1.5625、3.125、6.25、12.5和25μmol·L^(-1))对鸡原代成肌细胞增殖和分化的影响,并通过在7胚龄鸡胚卵黄囊部位注射不同浓度的VB2溶液(50、100和200μg),并设置0.9%的生理盐水为对照组,检测VB2对鸡胚胎骨骼肌发育的影响,试验共分为4组,每组25个胚蛋。研究结果表明,在细胞水平添加不同浓度的VB2都不同程度的促进了鸡原代成肌细胞的增殖活力,但是6.25μmol·L^(-1) VB2为最佳添加浓度。此外,添加6.25μmol·L^(-1) VB2可显著促进增殖标志基因CCND1(P<0.01)、PCNA(P<0.05)和CDK1(P<0.01)的表达,而对分化标志基因MYOD、MYOG和MYHC的表达无显著影响(P>0.05);同时,EdU试验也证实了在细胞水平添加6.25μmol·L^(-1) VB2可以显著促进成肌细胞的增殖(P<0.05)。此外,鸡胚卵黄囊部位注射VB2溶液试验表明注射50μg VB2后显著提高18胚龄鸡胚胸肌组织中增殖标志基因CDK1(P<0.01)、PCNA(P<0.001)及分化标志基因MYOD(P<0.05)、MYOG(P<0.05)的表达水平;同时,50μg VB2的添加也显著增加了18胚龄鸡胚胸肌肌纤维直径(P<0.05)和胸肌重(P<0.05)。上述结果表明,VB2可促进鸡原代成肌细胞的增殖作用和鸡胚的胸肌发育。因此,本研究结论表明VB2是影响鸡胚胸肌发育的一个重要的营养素,该研究为我国优质肉鸡早期胚胎营养调控提供重要的理论依据和应用价值。This study aimed to investigate the effect of riboflavin(also known as vitamin B2,VB2)on the development of skeletal muscle in chicken embryos to promote its application in embryo feeding.The CCK-8,EdU,and immunofluorescence detection methods were used to identify the effects of adding different concentrations of VB2(1.5625,3.125,6.25,12.5 and 25μmol·L^(-1))on the proliferation and differentiation of chicken primary myoblasts.Different concentrations of VB2 solution(50,100 and 200μg)were injected into the yolk sac of 7 embryonic age chicken embryos,and a 0.9%saline control group was set up to detect the effect of VB2 on the development of skeletal muscle in chicken embryos.The experiment was divided into 4 groups,with 25 embryo eggs in each group.The results showed that adding different concentrations of VB2 at the cellular level promoted the proliferation activity of chicken primary myoblasts to varying degrees,but 6.25μmol·L^(-1) VB2 was the optimal addition concentration.In addition,adding 6.25μmol·L^(-1) VB2 significantly promoted the expression of proliferation marker genes CCND1(P<0.01),PCNA(P<0.05),and CDK1(P<0.01),but had no significant effect on the expression of differentiation marker genes MYOD,MYOG,and MYHC(P>0.05);at the same time,EdU experiments also confirmed that adding 6.25μmol·L^(-1) VB2 at the cellular level could significantly promote the proliferation of myoblasts(P<0.05).In addition,the experiment of injecting VB2 solution into the yolk sac of chicken embryos showed that the injection of 50μg VB2 significantly increased the expression levels of proliferation marker genes CDK1(P<0.01),PCNA(P<0.001)and differentiation marker genes MYOD(P<0.05),MYOG(P<0.05)in the breast muscle tissue of 18 embryonic age chicken embryos;at the same time,the addition of 50μg VB2 also significantly increased the fiber diameter(P<0.05)and breast muscle weight(P<0.05)of the breast muscle of 18 embryonic age chicken embryos.The above results show that VB2 can promote the proliferation of chicken primary my

关 键 词：核黄素 骨骼肌发育 鸡 胚胎给养 

分 类 号：S831.5[农业科学—畜牧学]