母猪死胎和木乃伊全基因组关联分析  

Genome-wide Association Study of Stillbirths and Mummies in Sows

作　　者：周泰增 杨祎挺 朱悦华 钱洪喜 刘一辉 甘麦邻 朱砺[1,2,3] 沈林園 ZHOU Taizeng;YANG Yiting;ZHU Yuehua;QIAN Hongxi;LIU Yihui;GAN Mailin;ZHU Li;SHEN Linyuan(National Key Laboratory of Pig and Poultry Seed Industry,Sichuan Agricultural University,Chengdu 611130,China;Sichuan Provincial Key Laboratory of Exploration and Innovative Utilization of Livestock and Poultry Genetic Resources,Sichuan Agricultural University,Chengdu 611130,China;Key Laboratory of Livestock and Poultry Biomics of Ministry of Agriculture and Rural Affairs,College of Animal Science and Technology,Sichuan Agricultural University,Chengdu 611130,China;Tieqi Lishi Food Co.,Ltd.,Chengdu 610000,China;Sichuan Provincial Animal Husbandry Station,Chengdu 610066,China)

机构地区：[1]四川农业大学猪禽种业全国重点实验室,成都611130 [2]四川农业大学畜禽遗传资源发掘与创新利用四川省重点实验室,成都611130 [3]四川农业大学动物科技学院农业农村部畜禽生物组学重点实验室,成都611130 [4]铁骑力士食品有限责任公司,成都610000 [5]四川省畜牧总站,成都610066

出　　处：《畜牧兽医学报》2025年第3期1231-1241,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点研发计划(2021YFD1200801);四川省科技计划(2021YFYZ0030,2021ZDZX0008);国家现代农业产业技术体系四川生猪创新团队(SCCXTD-2024-8);国家生猪产业体系(CARS-35);中央引导地方科技发展资金项目(2023ZYDF056)。

摘　　要：旨在鉴定大白母猪初胎的死胎和木乃伊性状相关候选基因与遗传标记。本研究采用“中芯一号”50K SNP芯片对1805头大白母猪进行全基因组基因分型,经过数据结果的过滤与筛选,结合初胎死胎数和木乃伊数的表型数据,开展全基因组关联分析(GWAS)。研究结果表明,有24个SNPs与初胎死胎数、18个SNPs与木乃伊性状显著相关,并注释得到34个候选基因。其中,25个基因与死胎性状相关(如SIL1、FSTL4、DHX38、PLK2、PDE 4D等),9个基因与木乃伊性状相关(如LRRTM4、ERC2、ARHGEF3、U 6等)。通过猪QTL数据库(Pig QTLdb)进行比对分析发现,部分候选基因位于已有文献报道的与死胎和木乃伊性状相关的基因区域内,且显著SNPs存在连锁现象。GO和KEGG富集分析揭示了显著SNPs的上、下游注释基因与免疫反应、细胞信号传导、细胞凋亡与分裂以及疾病的产生等过程密切相关。此项研究筛选了大白猪群体的初胎死胎和木乃伊性状相关候选基因,并为分子育种提供重要参考。The aim of this research was to identify candidate genes and genetic markers associated with stillbirth and mummified fetus traits in the first litter of Large White sows.A total of 1805 Large White sows were genotyped using the"Zhongxin No.1"50K SNP chip.After filtering and selecting the data,a genome-wide association study(GWAS)was conducted,incorporating phenotypic data of stillbirths and mummified fetuses from the first litter.The results showed that 24 SNPs were significantly associated with the number of stillbirths,and 18 SNPs were significantly associated with mummified fetus traits,identifying a total of 34 candidate genes.Among these,25 genes were related to stillbirths(such as SIL1,FSTL4,DHX38,PLK2,PDE 4D,etc.),while 9 genes were associated with mummified fetus traits(including LRRTM4,ERC2,ARHGEF3,U 6,etc.).A comparison with the Pig QTL database(Pig QTLdb)revealed that some of the candidate genes were located within previously reported genomic regions associated with stillbirth and mummified fetus traits,and significant SNPs exhibited linkage disequilibrium.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses indicated that the genes annotated upstream and downstream of the significant SNPs were closely associated with biological processes such as immune responses,cell signaling,apoptosis and cell division,as well as disease development.This study screened the candidate genes related to the traits of stillbirth and mummy in Large White pig population,and provided an important reference for molecular breeding.

关 键 词：母猪 初胎 死胎数 木乃伊 GWAS SNP QTL 

分 类 号：S828.3[农业科学—畜牧学]