miRNA-424-5p和白细胞介素-6在结核菌不同感染状态中的诊断价值  

Diagnostic value of miRNA-424-5p and IL-6 in different infection status of Mycobacterium tuberculosis

作　　者：洪明阳 黄旭 王秋波 张海云 HONG Mingyang;HUANG Xu;WANG Qiubo;ZHANG Haiyun(Department of Clinical Laboratory,the Sixth People's Hospital of Nantong,Nantong,Jiangsu 226011,China;Nantong Institute of Genetics and Reproductive Medicine,the Affiliated Maternity and Child Healthcare Hospital of Nantong University,Nantong,Jiangsu 226018,China;Department of Clinical Laboratory,Wuxi Ninth People's Hospital,Wuxi,Jiangsu 214000,China;Department of Clinical Laboratory,Dalian Municipal Women and Children's Medical Center,Dalian,Liaoning 116012,China)

机构地区：[1]南通市第六人民医院检验科,江苏南通226011 [2]南通市妇幼保健院遗传与生殖医学研究所,江苏南通226018 [3]无锡市第九人民医院医学检验科,江苏无锡214000 [4]大连市妇女儿童医疗中心(集团)检验科,辽宁大连116012

出　　处：《中国热带医学》2024年第12期1524-1528,共5页China Tropical Medicine

基　　金：江苏省卫生健康员会2023年度医学科研立项面上项目(No.H2023093);南通市卫生健康委员会基金项目(No.QA2021051)

摘　　要：目的 探讨miRNA-424-5p和白细胞介素-6(interleukin-6, IL-6)在结核菌潜伏感染(latent tuberculosis infection, LTBI)患者和活动性结核(active tuberculosis, ATB)患者中的表达变化情况及其诊断价值。方法 登录美国国立生物技术信息基因表达综合中心(Gene Expression Omnibus, GEO)数据库下载微小核糖核酸(microRNA, miRNA)表达芯片GSE25435和GSE29190,使用GEO2R工具获取差异表达miRNA;选取2022年7月—2023年7月南通市第六人民医院收治的LTBI患者、ATB患者共30例,健康对照(healthy controls, HC)组为同时期健康体检者。采用实时荧光定量PCR(quantitative real-time PCR, qPCR)对3组人群外周血单个核细胞(peripheral blood mononuclear cell, PBMC)中miRNA和IL-6表达进行验证;通过受试者操作特征(receiver operating characteristic, ROC)曲线评估miRNA-424-5p和IL-6的诊断效能。结果 经GEO2R分析,获得3个差异miRNA,分别是miRNA-424-5p、miRNA-21-3p和miRNA-144-3p;使用qPCR实验对以上3个miRNA及IL-6的水平进行检测,发现与LTBI组相比,ATB组患者外周血单个核细胞中miRNA-21-3p和miRNA-144-3p的相对表达量无明显差异(F=6.53,P>0.05;F=3.21,P>0.05);但外周血单个核细胞中miRNA-424-5p和IL-6的表达水平出现明显升高,差异有统计学意义(F=5.35,P<0.01;F=8.77,P<0.01);ROC曲线分析结果显示,miRNA-424-5p和IL-6区分ATB与LTBI患者的最大曲线下面积(area under curve, AUC)值分别为0.70和0.76,两者联合诊断AUC值可达0.84。结论 miRNA-424-5p和IL-6的联合诊断具有区分LTBI和ATB患者的潜能,可为临床快速鉴别诊断潜伏感染和活动性结核提供一定的参考依据。Objective To investigate the changes in expression and diagnostic value of miRNA-424-5p and interleukin-6(IL-6)in patients with latent tuberculosis(LTBI)and active tuberculosis(ATB).Methods Data from the miRNA expression microarray GSE25435 and GSE29190 were downloaded from the U.S.National Center for Biotechnology Information Gene Expression Omnibus(GEO)database,and differentially expressed miRNA were obtained using the GEO2R tool.A total of 30 cases,including LTBI and ATB patients,were selected from the Sixth People's Hospital of Nantong between July 2022 to July 2023,with healthy controls(HC)being concurrent healthy individuals.The expression of miRNA and IL-6 in peripheral blood mononuclear cells(PBMCs)among three groups was verified by real-time fluorogenic quantitative PCR(qPCR).The diagnostic efficacy of candidate miRNA and IL-6 was evaluated by receiver operating characteristic(ROC)curves.Results By GEO2R analysis,three differential miRNAs were obtained:miRNA-424-5p,miRNA-21-3p,and miRNA-144-3p.qPCR experiments verified the levels of these three miRNAs and IL-6,showing that miRNA-21-3p and miRNA-144-3p exhibited no significant difference in relative expression in PBMCs of ATB patients compared to the LTBI group(F=6.53,P>0.05;F=3.21,P>0.05).However,the expression levels of miRNA-424-5p and IL-6 in PBMCs were significantly increased,with statistically significant differences(F=5.35,P<0.01;F=8.77,P<0.01).ROC curve analysis showed that the maximum AUC of miRNA-424-5p and IL-6 for distinguishing ATB from LTBI was 0.70 and 0.76 respectively,and their combined diagnostic AUC could reach 0.84.Conclusions The combined diagnosis of miRNA-424-5p and IL-6 has the potential to distinguish LTBI from ATB patients,providing a certain reference for the rapid differential diagnosis of latent infection and active tuberculosis.

关 键 词：潜伏性结核感染 活动性结核 诊断 微小核糖核酸 白细胞介素-6 

分 类 号：R521[医药卫生—内科学]