3株克斯特丛毛单胞菌特性研究  

Characterization of three strains of Comamonas kerstersii

作　　者：王崇圳 董灵芝 陈东科 欧阳汀兰 WANG Chongzhen;DONG Lingzhi;CHEN Dongke;OUYANG Tinglan(Department of Clinical Laboratory&Central Laboratory of Sanya People's Hospital,Sanya,Hainan 572000,China)

机构地区：[1]三亚市人民医院检验科/中心实验室,海南三亚572000

出　　处：《中国热带医学》2024年第12期1488-1493,共6页China Tropical Medicine

基　　金：海南省院士团队创新中心资金资助

摘　　要：目的 对3株克斯特丛毛单胞菌(Comamonas kerstersii)进行了一系列生化特性及基因分析,以增进对该菌的了解,为预防和控制与该菌相关的感染和疾病提供一定的参考依据。方法 三亚市人民医院2023年9月—10月3例腹痛腹泻患者粪便标本分别被接种于哥伦比亚血琼脂平板培养基,充分培养后对使用氧化酶试剂结果为阳性的菌株进行单菌落分离和纯培养。之后,使用VITEK2-Compact全自动微生物鉴定及药敏分析系统对目标菌落进行生化鉴定和抗生素药物敏感性的测定,使用VITEK MS质谱仪鉴定细菌所在菌种。同时,提取细菌DNA进行16S rRNA测序和细菌框架图测序,使用多种生物信息学分析工具进行测序分析。结果 本研究培养到了3株氧化酶阳性单菌落,且质谱仪无法鉴定出具体菌种。药敏试验结果表明3株细菌对大多数的抗菌药物敏感,但部分菌株对环丙沙星和复方新诺明耐药。16S rRNA测序将细菌鉴定到了Comamonas属,细菌框架图测序分析表明所有细菌均是克斯特丛毛单胞菌。生物信息学分析表明,3株细菌的毒力因子中均含有与氧化酶阳性的肠道致病菌相关的基因包括气单胞菌属的flmH基因、弧菌属的vctC基因和rmlB基因、耶尔森菌属的flgG基因和flhC基因。病原与宿主互作分析结果提示了目标菌基因组中可能存在引起肠道感染的病原基因。结论 克斯特丛毛单胞菌无法被常规质谱仪鉴定,需要借助测序展开分析。该菌对大多数抗菌药物敏感,但个别菌株对环丙沙星和复方新诺明耐药。克斯特丛毛单胞菌与人类感染,特别是肠道感染,存在一定的相关性,但致病机理有待后续研究。Objective This study conducted a series of biochemical and genetic analyses on three strains of Comamonas kerstersii isolated,aiming to enhance understanding of the bacterium and provide a reference for preventing and controlling infections and diseases associated with it.Methods The fecal samples of patients from three patients from September to October 2023 in Sanya People's Hospital were inoculated on Columbia blood agar plates,and after sufficient incubation,the target strains were isolated and purified using oxidase reagent.Subsequently,the target colonies were subjected to biochemical identification and antibiotic susceptibility testing using the VITEK2-Compact analysis system,while the VITEK MS mass spectrometer was used to identify the bacterial genus.Meanwhile,bacterial DNA was extracted for 16S rRNA sequencing and bacterial genome framework sequencing,with sequencing analysis performed using various bioinformatics analysis tools.Results Three strains of oxidase-positive single colonies were cultured,and the mass spectrometer could not identify the specific strain.The drug susceptibility test showed that all three strains were sensitive to most drugs,but some were resistant to ciprofloxacin and Compound Sulfamethoxazole.16S rRNA sequencing identified the bacteria to Comamonas genus,and bacterial genome framework sequencing analysis indicated that all bacteria were Comamonas kerstersii.Bioinformatics analysis showed that all three bacterial strains contained virulence factors related to oxidase-positive enteric pathogenic bacteria,including the flmH gene of Aeromonas,vctC gene and rmlB gene of Vibrio,and the flgG gene and flhC gene of Yersinia.Pathogen-host interaction(PHI)analysis results suggested pathogenic genes in the target bacterial genomes that may cause intestinal infection.Conclusions Comamonas kerstersii cannot be identified by conventional mass spectrometer and requires sequencing for analysis.This bacterium is sensitive to most antibiotics,but individual strains are resistant to ciprofloxacin

关 键 词：克斯特丛毛单胞菌 粪便 生化特征 毒力分析 PHI互作分析 

分 类 号：R446.5[医药卫生—诊断学]