性成熟前大鼠精索血管高位结扎对其性成熟后同侧睾丸的影响  

Effect of high ligation of spermatic vessels in pre-sexual maturity rats on ipsilateral testis at post-sexual maturity

作　　者：刘晓萌 苏哲 白东升[1] Liu Xiaomeng;Su Zhe;Bai Dongsheng(Department of Urology,Children's Hospital,Capital Institute of Pediatrics,Beijing 100020,China)

机构地区：[1]首都儿科研究所附属儿童医院泌尿外科,北京100020

出　　处：《中华小儿外科杂志》2025年第2期169-173,共5页Chinese Journal of Pediatric Surgery

基　　金：首都儿科研究所科研项目(LCYJ-2023-20)。

摘　　要：目的探讨性成熟前大鼠精索血管高位结扎对其性成熟后同侧睾丸的影响。方法选取性成熟前(30日龄)健康雄性SD大鼠16只,按数字随机法分为实验组(8只)和对照组(8只),实验组建立精索血管高位结扎模型,对照组仅高位游离出右侧精索血管。待其性成熟后(60日龄)摘取双侧睾丸和附睾,测量每只大鼠双侧睾丸长、宽、厚并计算睾丸体积;睾丸切片常规HE染色,显微镜下观察组织形态改变;采集每只大鼠双侧精液并计算精子浓度、精子活力及精子畸形率。比较组间(实验组手术侧与对照组手术侧)和组内(实验组手术侧与未手术侧、对照组手术侧与未手术侧)睾丸体积、精子浓度、精子活力及精子畸形率之间的差异。结果实验组手术侧和未手术侧睾丸体积分别为(1.06±0.99)cm^(3)和(2.18±0.17)cm^(3),对照组手术侧和未手术侧睾丸体积分别为(2.17±0.16)cm^(3)和(2.12±0.18)cm^(3)。实验组手术侧睾丸体积明显小于未手术侧和对照组手术侧(P=0.010和P=0.007),对照组手术侧与未手术侧睾丸体积差异无统计学意义(P=0.548)。实验组手术侧睾丸异常的生精小管数量增多,间距增大,管腔萎缩、塌陷,各级生精细胞数量减少,排列紊乱,部分腔内可见生精细胞脱落及空泡化形成;对照组手术侧睾丸曲细精管数量正常,各级生精细胞排列整齐,层次清楚,腔内可见大量精子细胞。实验组手术侧精子浓度、精子活力和精子畸形率分别为6.50(1.00,20.00)×10^(6)/ml、(4.75±4.46)%和(19.25±8.59)%,未手术侧精子浓度、精子活力和精子畸形率分别为39.00(25.75,89.25)×10^(6)/ml、(22.63±7.67)%和(14.38±5.29)%。对照组手术侧精子浓度、精子活力和精子畸形率分别为33.50(28.00,66.50)×10^(6)/ml、(17.13±12.41)%和(14.63±6.11)%,未手术侧精子浓度、精子活力和精子畸形率分别为39.50(25.50,68.75)×10^(6)/ml、(20.25±11.89)%和(9.25±3.61)%。实验组手术侧的精子浓�ObjectiveTo explore the effects of high ligation of spermatic vessels in pre-sexually mature rats on ipsilateral testis at post-sexual maturity.MethodsSixteen healthy male Sprague Dawley(SD)rats at pre-sexual maturity(30-day-old)were selected and randomly divided into an experimental group(eight rats)and a control group(eight rats).The experimental group underwent high ligation of spermatic vessels,while underwent sham surgery with only the right spermatic vessels being freed in the control group.Bilateral testes and epididymis were harvested for further analysis until these rats reached sexual maturity(60-day-old),and the length,width and thickness of each testis were measured and the testicular volume was calculated.Hematoxylin and eosin(H&E)staining was performed after testis tissue sectioning and morphological changes were observed under microscopic examination.Bilateral semen was collected from each rat and the sperm concentration,sperm motility and sperm deformity rate were calculated.The differences in testicular volume,sperm concentration,sperm motility and sperm malformation rate were compared between groups(operative side in experimental group and operative side in control group)and within groups(operative side and non-operative side in experimental group,operative side and non-operative side in control group).ResultsThe testicular volume on the operative and non-operative sides was 1.06±0.99 cm^(3)and 2.18±0.17 cm^(3),respectively in experimental group.In control group,the testicular volume was 2.17±0.16 cm^(3)and 2.12±0.18 cm^(3)on operative and non-operative sides,respectively.The testicular volume on the operative side of experimental group was significantly smaller than that on non-operative side and operative side of the control group(P=0.010 and P=0.007).There was no significant difference in testicular volume between operative side and non-operative side in the control group(P=0.548).In the experimental group,the number and distance of abnormal spermatogenic tubules significantly increased i

关 键 词：睾丸 精索血管高位结扎 性成熟前 组织病理学 

分 类 号：R726.9[医药卫生—儿科]