月见草丙酮提取物对脑缺血再灌注损伤的干预作用  

Interventional Effect of Acetone Extract of Oenothera biennis on Cerebral Ischemia-Reperfusion Injury

作　　者：张妍[1] 袁培培[1,2] 陈祎 郑亚娟 李赛菲 赵李蕊 郑晓珂 冯卫生[1,2,3] ZHANG Yan;YUAN Peipei;CHEN Yi;ZHENG Yajuan;LI Saifei;ZHAO Lirui;ZHENG Xiaoke;FENG Weisheng(Henan University of Chinese Medicine,Zhengzhou 450046;Engineering and Technology Center for Chinese Medicine Development of Henan Province,Zhengzhou 450046;Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-Constructed by Henan Province&Education Ministry of P.R.China,Zhengzhouu450046)

机构地区：[1]河南中医药大学,郑州450046 [2]河南省中药开发工程技术研究中心,郑州450046 [3]呼吸疾病中医药防治省部共建协同创新中心,郑州450046

出　　处：《中药药理与临床》2025年第1期94-100,共7页Pharmacology and Clinics of Chinese Materia Medica

基　　金：中央引导地方科技发展专项河南道地大宗药材种质评价及集约化种植与示范(编号:豫财科[2016]149号)。

摘　　要：目的:研究月见草提取物对脑缺血再灌注损伤的干预作用,为月见草进一步开发利用提供理论依据。方法:将雄性SD大鼠随机分为假手术对照组、模型对照组、月见草提取物88、175 mg/kg组和阳性药尼莫地平10 mg/kg组,每组10只。采用动脉闭塞/再灌注模型(Middle cerebral artery occlusion/reperfusion, MCAO/R)建立大鼠脑缺血再灌注损伤模型,采用Longa 5评分法进行神经功能评分;2,3,5-氯化三苯基四氮唑(TTC)染色评价脑梗死体积;苏木精-伊红(HE)染色观察大脑皮质神经细胞损伤;尼氏(Nissl)染色观察脑组织神经元损伤情况;黄嘌呤氧化酶法和硫代巴比妥酸法分别测定血清超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量;紫外分光光度法和酶标法分别测定血清谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活力;流式细胞术检测脑细胞活性氧(ROS)和凋亡水平。结果:与假手术对照组相比,模型对照组大鼠神经功能评分、大脑系数、脑梗死范围、MDA含量、ROS及凋亡水平显著升高(P<0.01),SOD、GSH-Px和CAT活力显著降低(P<0.01),大脑皮质细胞形态以及海马CA1区神经元细胞出现损伤;与模型对照组相比,月见草提取物各组神经功能评分、大脑系数、脑梗死范围、MDA含量、ROS及凋亡水平显著降低(P<0.01),SOD、GSH-Px和CAT活力明显升高(P<0.05或P<0.01),大脑皮质细胞形态以及海马CA1区神经元细胞损伤显著改善。结论:月见草提取物通过改善氧化应激、降低细胞凋亡水平,保护神经功能,发挥对脑缺血再灌注损伤的干预作用。Objective:To study the intervention effect of Oenothera biennis extract on cerebral ischemia-reperfusion injury to provide a theoretical basis for the further development and utilization of O. biennis. Methods:Male SD rats were randomly divided into sham operation control group, model control group, O. biennis extract groups at doses of 88 mg/kg and 175 mg/kg, and a positive drug Nimodipine group(10 mg/kg),with 10 rats in each group. A middle cerebral artery occlusion/reperfusion(MCAO/R) model was used to establish an in vivo cerebral ischemia-reperfusion injury model. Neurological function was assessed using the Longa 5-point scoring method. Infarct volume was evaluated by 2,3,5-triphenyltetrazolium chloride(TTC) staining. Cortical neuronal damage was observed using hematoxylin-eosin(HE) staining. Neuronal damage in brain tissue was assessed by Nissl staining. Serum superoxide dismutase(SOD) activity and malondialdehyde(MDA) levels were measured using xanthine oxidase method and thiobarbituric acid method, respectively. Serum glutathione peroxidase(GSH-Px) activity and catalase(CAT) activity were measured by UV spectrophotometry and enzyme-linked assay, respectively. Reactive oxygen species(ROS) and apoptosis levels in brain cells were detected by flow cytometry. Results:In vivo experiments showed that, compared with the sham operation control group, the model control group had significantly higher neurological function scores, brain coefficient, infarct size, MDA levels, ROS,and apoptosis levels(P<0.01),significantly lower SOD,GSH-Px, and CAT activities(P<0.01),and damaged cortical cell morphology and hippocampal CA1 neuronal cells. Compared with the model control group, the O. biennis extract groups showed significantly lower neurological function scores, brain coefficient, infarct size, MDA levels, ROS,and apoptosis levels(P<0.01),significantly higher SOD,GSH-Px, and CAT activities(P<0.05 or P<0.01),and significantly improved cortical cell morphology and hippocampal CA1 neuronal cell damage. Conclusion:O. bie

关 键 词：月见草提取物 脑缺血再灌注损伤 神经保护 氧化应激 凋亡 

分 类 号：R285.5[医药卫生—中药学]