黄瓜CsCLE3基因克隆及表达分析  

Cloning and Expression Analysis of CsCLE3 Gene in Cucumber

作　　者：雷明月 张瑶 宋晓飞[1] 李晓丽[1] 孙成振[1] 闫立英[1] Lei Mingyue;Zhang Yao;Song Xiaofei;Li Xiaoli;Sun Chengzhen;Yan Liying(Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization,College of Horticulture Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao,066004)

机构地区：[1]河北科技师范学院园艺科技学院,河北省特色园艺种质挖掘与创新利用重点实验室,秦皇岛066004

出　　处：《分子植物育种》2025年第2期396-401,共6页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31801877);河北省高等学校科学技术研究项目(BJ2018002);河北省二期现代农业产业技术体系创新团队项目(HBCT2018030209);国家级大学生创新创业训练计划项目(202110798001)共同资助。

摘　　要：以黄瓜(Cucumis sativus L.)自交系6457花冠为材料,采用RT-PCR技术克隆了黄瓜Cs CLE3基因。通过生物信息学分析,获得黄瓜CsCLE3基因CDS全长为264 bp,无内含子,编码87个氨基酸;蛋白质分子质量为9737.34 Da,等电点为9.39;CsCLE3含有保守的CLE motif序列,是一个典型的CLE家族基因;系统进化树分析表明Cs CLE3与木槿同源蛋白的亲缘关系最近。通过荧光定量PCR分析,发现Cs CLE3基因表达量随着花冠开放逐渐升高,黄蕾期达到最高,在开花当天表达量迅速下降,呈现先升高后降低的变化趋势,但在超级子房花冠中的表达水平始终显著低于正常子房。本研究推测Cs CLE3基因可能参与调控黄瓜超级子房的形成过程,以期为超级子房调控的机理研究提供理论依据。The corolla of cucumber(Cucumis sativus L.)self-line 6457 was used as the research material,CsCLE3 gene was cloned by RT-PCR.Through bioinformatics analysis,the full-length CDS of cucumber CsCLE3gene was 264 bp,without introns and encoded 87 amino acids.The molecular weight of protein was 9737.34 Da,and the isoelectric point was 9.39;CsCLE3 contains a conserved CLE motif sequence,which is a typical CLE family gene;phylogenetic tree analysis indicated that CsCLE3 had the closest relationship with the homologous protein of Hibiscus syriacus.The results of real-time PCR showed that the expression level of Cs CLE3 gene gradually increased with the opening of corolla,and reached the highest level at the yellow bud stage.The expression level rapidly decreased on the day of flowering,showing a trend of first increase and then decrease,but the expression level in the super ovary corolla was always significantly lower than the normal ovary.This study hypothesizes that the CsCLE3 gene may be involved in regulating the process of forming super-ovaries in cucumber,with the aim of providing theoretical basis for the study of the mechanism of super-ovary regulation.

关 键 词：黄瓜 超级子房 CsCLE3 基因克隆 表达分析 

分 类 号：S64[农业科学—蔬菜学]