基于GC-MS技术分析NaCl胁迫越橘小分子代谢物变化  

Analysis of the Changes of Small Molecular Metabolites of Blueberry under NaCl Stress Based on GC-MS Technology

作　　者：高龙飞 张卫华 田立国[2] 贾斌 李天杰 杨柏明 李猛[2] 刘颖 刘海学 吴颖 Gao Longfei;Zhang Weihua;Tian Liguo;Jia Bin;Li Tianjie;Yang Baiming;Li Meng;Liu Ying;Liu Haixue;Wu Ying(College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin,300392;College of Automation and Electrical Engineering,Tianjin University of Technology and Education,Tianjin,300222;Changchun Guoxin Modern Agricultural Technology Development Co.,Ltd.,Changchun,130000)

机构地区：[1]天津农学院园艺园林学院,天津300392 [2]天津职业技术师范大学自动化与电气工程学院,天津300222 [3]长春国信现代农业科技发展股份有限公司,长春130000

出　　处：《分子植物育种》2025年第2期574-582,共9页Molecular Plant Breeding

基　　金：国家自然科学基金面上项目(61971312);天津农学院研究生科研创新资助项目(2019XY013);天津市高等学校学科领军人才、天津市创新类领军人才引进专项共同资助。

摘　　要：为探寻不同浓度NaCl盐胁迫下越橘苗期叶片小分子代谢物质的变化,本研究以越橘2年生幼苗为材料,设置盐胁迫(NaCl)溶液浓度分别为50、100和200 mmol/L,正常去离子水浇灌设为对照组(CK),在盐处理16 d取样,样品两两分组,组成L_(1)~L_(4)共4个处理组,采用GC-MS技术对4组样品进行代谢产物对比分析。4个处理组的差异代谢物数量分别为32、39、33和37种,其中D-果糖等14个代谢物为4个处理组中重复出现的差异代谢物。聚类热图分析发现L_(1)、L_(2)和L_(4)组中氨基酸类、碳水化合物类和有机酸类代谢物以上调为主,有机化合物类代谢物以下调为主;L_(3)组各类代谢物均以下调为主。差异代谢物KEGG注释显示,L_(1)~L_(4)组共注释到17条差异显著的代谢通路,其中有13条注释到氨基酸类和碳水化合物类代谢通路,表明这2类代谢通路在越橘抵御盐胁迫中占主导地位。以上结果为深入研究越橘的盐胁迫应答机制提供了试验基础。In order to explore the changes of small molecular metabolites in the leaves of blueberry seedlings under different concentrations of Na Cl salt stress,in this study,2-year-old blueberry seedlings were used as materials,the salt stress(NaCl)solution concentration was set to 50,100,and 200 mmol/L,and normal deionized water irrigation was set as the control group(CK).Samples were taken on 16 days of salt treatment.The samples were grouped in pairs to form a total of 4 treatment groups from L_(1)to L_(4).The metabolites of the 4 groups of samples were compared and analyzed by GC-MS technology.The number of differential metabolites in the 4 treatment groups were32,39,33,and 37,respectively.Among them,14 metabolites such as D-fructose were the differential metabolites repeated in the 4 treatment groups.Clustering heat map analysis found that the metabolites of amino acids,carbohydrates and organic acids in the L_(1),L_(2)and L_(4)groups were mainly up-regulated,and the metabolites of organic compounds were mainly down-regulated;all metabolites of the L_(3)group were all down-regulated to host.The KEGG annotation of differential metabolites showed that there were a total of 17 significantly different metabolic pathways annotated in the L_(1)~L_(4)groups,13 of which were annotated to amino acid and carbohydrate metabolic pathways,indicating that these two types of metabolic pathways played a dominant role in the resistance of blueberry to salt stress.The result of this study provides an experimental basis for in-depth study of the salt stress response mechanism of blueberry.

关 键 词：越橘 NACL胁迫 GC-MS 小分子代谢物 

分 类 号：S663.9[农业科学—果树学]