钙离子对高脂饮食小鼠肠道菌群的影响  

Effects of Calcium Ions on the Gut Microbiota of Mice Fed a High-Fat Diet

作　　者：马春丽 赵林昀 扈瑞平[1] 李丽[1] 曹丽丽 红梅[1] 包玉龙[1] MA Chunli;ZHAO Linyun;HU Ruiping;LI Li;CAO Lili;Hongmei;BAO Yulong(Inner Mongolia Medical University,Hohhot010107,China)

机构地区：[1]内蒙古医科大学,内蒙古呼和浩特010107

出　　处：《畜牧与饲料科学》2025年第1期1-9,共9页Animal Husbandry and Feed Science

基　　金：2022年度内蒙古自治区卫生健康科技计划项目(202202136);内蒙古自治区高等学校青年科技英才支持计划(NJYT23052);内蒙古自治区高等学校创新团队发展计划(NMGIRT2418);内蒙古医科大学面上项目校级面上项目(YKD2022MS006);内蒙古医科大学博士启动基金项目(YKD2023BSQD004);内蒙古自治区蒙医药协同创新中心成果转化培育项目(MYYXTPY202309)。

摘　　要：[目的]探究钙离子对高脂饮食小鼠肠道菌群的影响。[方法]将30只6~8周龄的雄性C57bL/6J小鼠随机分为3组,分别为标准饮食对照组(C组)、高脂饮食组(M组)和高脂饮食加氯化钙处理组(D组),每组10只小鼠。C组给予标准饲料+普通饮用水,M组给予高脂饲料+普通饮用水,D组给予高脂饲料+添加1.5 g/100 mL的氯化钙的饮用水,所有小鼠连续饲喂11周后,每组小鼠随机选择3只小鼠采集粪便样本。运用16S rRNA高通量测序技术对肠道菌群进行测序,而后利用Perl和R编程语言计算物种丰富度、均匀度和多样性,比较样本间共有与特有OTU;使用QIIME软件分析α多样性(Shannon指数)和丰富度(ACE、Chao1指数);使用R语言STATS包的Wilcox检验及LDA直方图分析组间菌群差异;利用PICRUSt软件分析16S rRNA测序数据获取的物种组成信息,结合KEGG数据库对各组微生物群落的功能基因进行分类和富集差异分析。[结果]①3组的9个样本测序共获得953922个有效序列;ACE指数、Chao1指数和Shannon指数分析,各组间物种丰富度和多样性无显著差异(P>0.05)。②属和种水平上的物种相对丰度分析发现,C组、M组和D组的优势属分别为杜氏杆菌属(Dubosiella)、Lachnospiraceae NK4A136 group和异杆菌属(Ileibacterium),相对丰度分别为14.34%、14.49%、21.69%;C组、M组和D组的优势种分别为Clostridiales bacterium CIEAF 020、瓦伦斯回肠杆菌(Ileibacterium valens)、Ileibacterium valens,相对丰度分别为1.36%、7.24%、21.69%。③不同组别菌群差异分析表明,D组菌群与C组和M组不同,主要为Ileibacterium、Ileibacterium valens、变形菌门(Proteobacteria)、芽孢杆菌纲(Bacilli)、丹毒丝菌目(Erysipelotrichales)、丹毒丝菌科(Erysipelotrichaceae)。④PICRUSt预测功能基因组成显示,D组在碳固定途径、丙酮酸代谢和淀粉蔗糖代谢等功能基因通路上与M组存在显著差异(P<0.05),提示氯化钙处理可能影响了这些代谢途径�[Objective]To investigate the effects of calcium ions on the gut microbiota of mice fed a high-fat diet.[Methods]Thirty 6-to 8-week-old male C57BL/6J mice were randomly divided into three groups:a standard diet control group(C group),a high-fat diet group(M group),and a high-fat diet supplemented with calcium chloride treatment group(D group),with 10 mice in each group.The C group was fed a standard diet and given regular drinking water,the M group was fed a high-fat diet and given regular drinking water,and the D group was fed a high-fat diet and given drinking water supplemented with 1.5 g/100 mL calcium chloride.After 11 weeks of continuous feeding,fecal samples were collected from each group.The gut microbiota was sequenced using 16S rRNA high-throughput sequencing technology.Species richness,evenness,and diversity were calculated using Perl and R programming languages,and shared and unique OTUs were compared among samples.Alpha diversity(Shannon index)and richness(ACE and Chao1 indices)were analyzed using QIIME software.Differences in microbiota between groups were analyzed using the Wilcox test and LDA histogram from the R STATS package.The PICRUSt software was used to analyze the species composition information obtained from the 16S rRNA sequencing data,and in combination with the Kyoto Encyclopedia of Genes and Genomes(KEGG)database,the functional genes of the microbial communities in each group were classified and analyzed for enrichment differences.[Results]①A total of 953922 valid sequences were obtained from the sequencing of nine samples across the three groups.Analysis of the ACE index,Chao1 index,and Shannon index showed no significant differences in species richness and diversity among the groups(P>0.05).②Analysis of species relative abundance at the genus and species levels revealed that the dominant genera in the C,M,and D groups were Dubosiella,Lachnospiraceae NK4A136 group,and Ileibacterium,with relative abundances of 14.34%,14.49%,and 21.69%,respectively.The dominant species in the C,M,an

关 键 词：钙离子 肥胖 肠道菌群 16S rRNA高通量测序 小鼠 

分 类 号：R589.9[医药卫生—内分泌]