云南省玉溪市羊棘球蚴病血清抗体调查及分析  

Serological Survey and Analysis of Echinococcosis in Sheep in Yuxi City,Yunnan Province

作　　者：李晶 吕嵘 宋诗雅 杨秋楠 朱禹坤 张晓舟 杨曦 吴明伟 罗晓燕 徐聪 LI Jing;LYU Rong;SONG Shiya;YANG Qiunan;ZHU Yukun;ZHANGXiaozhou;YANG Xi;WU Mingwei;LUO Xiaoyan;XU Cong(Yuxi Center for Animal Health Supervision and Disease Control,Yuxi653100,China;Yuxi Animal Husbandry and Fishery Development Service Center,Yuxi653100,China)

机构地区：[1]玉溪市动物卫生监督与疫病预防控制中心,云南玉溪653100 [2]玉溪市畜牧渔业发展服务中心,云南玉溪653100

出　　处：《畜牧与饲料科学》2025年第1期109-114,共6页Animal Husbandry and Feed Science

基　　金：国家自然科学基金项目(32260889);云南省国际科技特派员项目(202403AK140020);云南省科技特派员项目(202204BK090769)。

摘　　要：[目的]了解云南省玉溪市羊棘球蚴病的流行情况,为该地区科学防控羊棘球蚴病提供参考。[方法]采集玉溪市江川区、澄江市、华宁县、易门县、峨山彝族自治县、元江哈尼族彝族傣族自治县6个区(市、县及民族自治县)非免疫羊血清304份,利用ELISA方法检测棘球蚴病血清抗体,分析不同采样地点、饲养规模、年龄段、饲养模式、品种、性别羊棘球蚴病血清抗体阳性率的统计学差异。[结果]在304份羊血清样品中,检出阳性样品14份,总抗体阳性率为4.61%。江川区羊棘球蚴病血清抗体阳性率最高(7.50%),澄江市羊棘球蚴病血清抗体阳性率(0)最低,不同区(市、县及民族自治县)羊棘球蚴病血清抗体阳性率差异不显著(P>0.05);散养羊血清抗体阳性率(7.14%)高于规模场羊阳性率(2.00%),差异显著(P<0.05);≥3岁羊血清抗体阳性率最高(8.57%),≤1岁羊阳性率最低(0.95%),不同年龄段羊血清抗体阳性率差异显著(P<0.05);小群放牧羊血清抗体阳性率最高(8.06%),舍饲羊阳性率最低(1.20%),不同饲养模式羊血清抗体阳性率差异显著(P<0.05);绵羊血清抗体阳性率(9.41%)高于本地山羊阳性率(2.74%),二者差异显著(P<0.05);公羊血清抗体阳性率(6.00%)略高于母羊阳性率(4.33%),二者差异不显著(P>0.05)。[结论]参与调查的玉溪市江川区、澄江市、华宁县、易门县、峨山彝族自治县、元江哈尼族彝族傣族自治县6个区(市、县及民族自治县)中除澄江市未检出阳性样本,其余5个区(市、县及民族自治县)均存在羊棘球蚴感染,散养羊、高年龄段羊、小群放牧羊和绵羊的棘球蚴感染率较高。应采取综合防控措施,有效降低羊棘球蚴感染率。[Objective]To investigate the prevalence of echinococcosis in sheep in Yuxi City,Yunnan Province,to provide a scientific basis for its prevention and control in the region.[Methods]A total of 304 serum samples from non-immunized sheep were collected from six districts,cities,counties,or ethnic autonomous counties in Yuxi City,including Jiangchuan District,Chengjiang City,Huaning County,Yimen County,Eshan Yi Autonomous County,and Yuanjiang Hani,Yi and Dai Autonomous County.The enzyme-linked immunosorbent assay(ELISA)was employed to detect serum antibodies against Echinococcus spp.,and statistical differences in antibody positivity rates were analyzed across various factors,including sampling locations,farming scales,age groups,rearing modes,breeds,and sexes.[Results]Of the 304 sheep serum samples,14 tested positive,yielding an overall antibody positivity rate of 4.61%.Jiangchuan District exhibited the highest positivity rate(7.50%),while Chengjiang City showed the lowest(0),with no significant differences in positivity rates among the districts,cities,counties,and autonomous counties(P>0.05).The positivity rate in free-range sheep(7.14%)was higher than that in large-scale farm sheep(2.00%),with significant differences(P<0.05).Sheep aged≥3 years had the highest positivity rate(8.57%),while those aged≤1 year had the lowest(0.95%),with significant differences across age groups(P<0.05).Small-scale grazing sheep showed the highest positivity rate(8.06%),while barn-fed sheep had the lowest(1.20%),with significant differences among rearing modes(P<0.05).Sheep exhibited a higher positivity rate(9.41%)than local goats(2.74%),with a significant difference between breeds(P<0.05).The positivity rate in male sheep(6.00%)was slightly higher than in female sheep(4.33%),though the difference was not significant(P>0.05).[Conclusion]With the exception of Chengjiang City,where no positive samples were detected,echinococcosis infection was confirmed in sheep across the remaining five districts,cities,counties,or autonomous counti

关 键 词：玉溪市 羊 棘球蚴病 ELISA检测 阳性率 血清抗体 

分 类 号：S858.26[农业科学—临床兽医学] S858.27[农业科学—兽医学]