低氧环境对妊娠大鼠胎盘组织TNF-α、IL-10的表达及其生殖功能、胚胎发育的影响  

Effects of hypoxia on expression of TNF-αand IL-10 in placental tissue,reproductive function and embryonic development of pregnant rats

作　　者：李丹 李建华 许佳佳 Li Dan;Li Jianhua;Xu Jiajia(Department of Human Anatomy,Medical College of Qinghai University,Xining 810016,China)

机构地区：[1]青海大学医学院人体解剖学教研室,西宁810016

出　　处：《解剖学杂志》2025年第1期7-11,F0002,共6页Chinese Journal of Anatomy

基　　金：青海省科技厅项目(2019-SF-134)。

摘　　要：目的:探讨高原低氧环境下妊娠大鼠体内,肿瘤坏死因子α(TNF-α)、白介素10(IL-10)在胎盘中的表达及对生殖功能、子代发育的影响。方法:将20只雌鼠、10只雄鼠以2∶1为一笼随机分为低氧组(低压氧舱,模拟6000 m海拔)和常氧组(青海省西宁市,平均2200 m海拔)饲养,记录每组雌鼠受孕率,直至妊娠第20天,2组妊娠大鼠禁食12h后,尾部采血测量空腹血糖,采取腹主动脉血液、取整个子宫,并剥离胎盘和胎鼠。记录2组妊娠大鼠子宫质量、胎鼠数量,计算2组妊娠大鼠子代畸形率。ELISA检测妊娠大鼠血清空腹胰岛素水平,H-E染色观察低氧下胎盘组织结构的改变,免疫组织化学染色检测妊娠大鼠胎盘组织TNF-α、IL-10阳性表达情况,RT-qPCR及免疫印迹检测妊娠大鼠胎盘组织TNF-α、IL-10mRNA及蛋白表达。结果:与常氧组相比,低氧组妊娠大鼠孕晚期子宫质量较轻;低氧组妊娠大鼠空腹血糖降低、空腹胰岛素水平升高;低氧组妊娠大鼠受孕率降低,胎鼠数量减少,胎鼠畸形率升高。低氧组妊娠大鼠胎盘结构不完整,血管分布增多,管壁增厚,绒毛发育缓慢且数目减少,绒毛间质血管分布密集,基底膜增厚,可见少量炎症细胞,合体滋养层增厚界限模糊。与常氧组比较,低氧组胎盘组织中TNF-α、IL-10 mRNA和蛋白表达均上调。结论:低氧环境下胎盘组织中的TNF-α、IL-10表达上调,可能与雌性大鼠生殖功能下降、不良妊娠有关。Objective:To investigate the expression of tumor necrosis factor-alpha(TNF-α)and interleukin-10(IL-10)in the placenta under high-altitude hypoxic conditions,and the effects on reproductive function and offspring development.Methods:A total of 20 female rats and 10 male rats were randomly divided into hypoxic group(simulated 6000 m altitude in a hypobaric oxygen chamber)and normoxia group(Xining city,Qinghai province,with an average altitude of 2200 m)with a female-to-male ratio of 2:1 per cage.The pregnancy rate of female rats in each group was recorded.On the 20th day of gestation,after a 12-hour fast,blood samples were collected from the tails of the pregnant rats to measure their fasting glucose.Blood was also taken from the abdominal aorta,and the entire uterus was excised to isolate the placenta and fetuses.The uterine weight and number of fetal rats were recorded,and the offspring malformation rate was calculated for both groups.ELISA was used to detect the serum fasting insulin level of pregnant rats,and H-E staining was used to observe the alteration of placental tissues in hypoxia.Immunohistochemistry was utilized to detect the positive expression of TNF-αand IL-10 in the placental tissues of pregnant rats.RT-qPCR and Western blotting were employed to detect the mRNA and protein expression of TNF-αand IL-10 in placental tissues of pregnant rats.Results:Compared with the normoxia group,the hypoxia group showed significantly lower uterine weight in pregnant rats during late pregnancy,reduced fasting blood glucose and increased fasting insulin levels,lower conception rates,fewer fetuses,and a higher rate of fetal malformations.The placenta of pregnant rats in the hypoxia group was structurally incomplete,with increased distribution of blood vessels,thickened vessel wall,slow development and reduced number of chorionic villi,densely distributed intervillous blood vessels,thickened basement membrane,a small number of inflammatory cells,and blurred boundaries of thickened syncytial trophoblast.Compared with

关 键 词：高原低氧 妊娠大鼠 肿瘤坏死因子α 白介素10 生殖功能 胎盘 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]