转录因子AP-1参与孕酮下调蜕膜基质细胞IL-8表达对早期自然流产的影响  

Transcription factor AP-1 mediates progesterone-induced downregulation of IL-8 expression in decidual stromal cells and its effect on early spontaneous abortion

作　　者：宋芳[1] 李永红 李娜 栾兆进 赵紫薇[1] 宫晓玲 杨美霞[1] Song Fang;Li Yonghong;Li Na;Luan Zhaojin;Zhao Ziwei;Gong Xiaoling;Yang Meixia(Department of Histology and Embryology,Baotou Medical College,Baotou 014040;Department of Histology and Embryology,Jinzhou Medical University,Jinzhou 121000;The Third Hospital of Baogang Group,Baotou 014040,China)

机构地区：[1]包头医学院组织学与胚胎学教研室,包头014040 [2]锦州医科大学组织学与胚胎学教研室,锦州121000 [3]包钢第三职工医院,包头014040

出　　处：《解剖学杂志》2025年第1期12-16,33,共6页Chinese Journal of Anatomy

基　　金：内蒙古自治区自然科学基金(2021MS08056,2022MS08037,2023MS08063);内蒙古自治区卫生健康科技计划项目(202201393);包头医学院博士启动基金(BSJJ202205);包头医学院创新团队发展计划项目(bycxtd-03)。

摘　　要：目的:探究孕酮经转录因子激活蛋白1(AP-1)对蜕膜基质细胞(DSCs)白细胞介素-8(IL-8)表达的调节作用。方法:采用免疫组织化学染色和免疫印迹检测流产组和对照组蜕膜组织AP-1的表达量;给予体外培养人DSCs不同浓度孕酮和AP-1抑制剂SR-11302处理,免疫印迹检测AP-1和p-AP-1的表达量;RT-qPCR和免疫印迹检测IL-8表达量。结果:p-AP-1在流产组蜕膜组织中的表达量高于对照组;0.1μmol/L和1μmol/L孕酮组细胞p-AP-1表达量明显低于对照组;孕酮组IL-8mRNA表达水平明显低于对照组,孕酮+SR-11302组中IL-8mRNA表达水平明显低于孕酮组;与对照组相比,孕酮组IL-8和p-AP-1蛋白表达量均降低;与孕酮组相比,孕酮+SR-11302组中IL-8和p-AP-1蛋白表达量均降低。结论:孕酮通过AP-1下调DSCs中IL-8的表达,为其用于防治促炎细胞因子表达异常所致的早期自然流产提供一定的理论依据。Objective:To explore the regulatory effect of progesterone on the expression of interleukin-8(IL-8)in decidual stromal cells(DSCs)through transcription factor activator protein 1(AP-1).Methods:Immunohistochemical staining and western blotting(WB)were used to detect the expression level of AP-1 protein in decidual tissue of the abortion group and the control group.Early pregnancy human DSCs were isolated and cultured in vitro,and cells were treated with different concentrations of progesterone(0.01μmol/L,0.1μmol/L,and 1μmol/L).WB was used to detect the expression of AP-1 and p-AP-1 in DSCs.After AP-1 inhibitor SR-11302 treatment,the expression of IL-8 in the inhibitor group,progesterone group,and control group was detected by using RT-qPCR and WB.Results:The expression level of p-AP-1 protein in decidual tissue of the abortion group was higher than that of the control group;the relative expression levels of p-AP-1 protein in the 0.1μmol/L and 1μmol/L progesterone group were significantly lower than that in the control group;IL-8 mRNA levels were significantly lower in the progesterone group compared with the control group,and were further reduced in the progesterone+SR-11302 group compared with the progesterone group;compared with the control group,the relative expression levels of IL-8 and p-AP-1 proteins in the progesterone group were reduced,and compared with the progesterone group,the relative expression levels of IL-8 and p-AP-1 proteins in the progesterone+SR-11302 group were also reduced.Conclusions:Progesterone downregulates the expression of IL-8 in DSCs via AP-1,offering a theoretical basis for its use in preventing and treating early spontaneous abortion induced by abnormal expression of proinflammatory cytokines.

关 键 词：激活蛋白1 白细胞介素-8 蜕膜基质细胞 孕酮 早期自然流产 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]