GBP3依赖其GTP酶活性负向调控HTLV-1病毒复制  

GBP3 negatively regulates HTLV-1 replication in a GTPase-dependent manner

作　　者：刘燕子 赵鑫[1] 秦潇[2] 黄雨璐 杨曦 范晴晴 杨波[2] 王洁[2] LIU Yanzi;ZHAO Xin;QIN Xiao;HUANG Yulu;YANG Xi;FAN Qingqing;YANG Bo;WANG Jie(Department of Clinical Laboratory,the Third Affiliated Hospital of Xinxiang Medical University,Xinxiang 453003,China;School of Medical Technology,Xinxiang Medical University,Xinxiang 453003,China)

机构地区：[1]新乡医学院第三附属医院检验科,新乡医学院医学技术学院,新乡453003 [2]新乡医学院医学技术学院,新乡453003

出　　处：《中国免疫学杂志》2025年第3期535-539,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(32070949)。

摘　　要：目的:探讨GBP3对成人T淋巴细胞白血病1型病毒(HTLV-1)复制的影响。方法:采用Western blot检测HTLV-1感染的HeLa细胞和THP1细胞中GBP3的表达量;在THP1和HeLa细胞中通过siRNA沉默GBP3,Western blot验证GBP3敲低效率;过表达GBP3或敲减GBP3后,采用RT-qPCR和Western blot检测HTLV-1感染细胞中前病毒转录本Tax、px、HBZ、Gag、ENV、5´UTR和病毒蛋白Tax和p19蛋白的表达量;构建失去GTP酶活性的GBP3的突变体GBP3K51A,检测GBP3对HTLV-1感染的调控是否依赖于GBP3的GTP酶活性。结果:HTLV-1感染的HeLa和THP1细胞中GBP3表达上升;GBP3过表达可导致HTLV-1的前病毒转录本和病毒蛋白表达量下降,siRNA敲减GBP3则具有相反的效果,GBP3K51A过表达可导致HTLV-1前病毒转录本和病毒蛋白表达量增加。结论:HTLV-1病毒感染可诱导GBP3表达;GBP3过表达抑制病毒复制,可能依赖GTP酶活性。Objective:To investigate the effect of GBP3 on replication of adult T-lymphocytic leukemia virus type 1(HTLV-1).Methods:Expression of GBP3 in HTLV-1-infected HeLa cell and THP1 cell was detected by Western blot.The knock-down efficiency of siRNAs targeting GBP3 in HeLa and THP1 cells was evaluated by Western blot.Effects of GBP3 overexpression or knockdown on expression of HTLV-1 proviral transcripts Tax,px,HBZ,Gag,ENV,5´UTR,and viral proteins Tax,p19 were investigated by RTqPCR and Western blot.GTPase-defective mutant of GBP3,GBP3K51A was constructed to explore whether the effects of GBP3 on HTLV-1 infection were dependent on its GTPase activity.Results:GBP3 expression was upregulated in HTLV-1 infected HeLa and THP1 cells.GBP3 overexpression decreased expression of HTLV-1 proviral transcripts and viral proteins,whereas the knockdown of GBP3 has the opposite effects.Overexpression of GBP3K51A increased expression of HTLV-1 proviral transcripts and viral proteins.Conclusion:HTLV-1 virus infection can induce expression of GBP3;overexpression of GBP3 inhibits virus replication and may depend on GTPase.

关 键 词：GBP3 HTLV-1 抗病毒 GTP酶活性 

分 类 号：R392.1[医药卫生—免疫学]