苦参碱通过调控miR-125b-5p/STAT3途径抑制人脐静脉内皮细胞增殖  

Matrine inhibits proliferation of human umbilical vein endothelial cells by regulating miR-125b-5p/STAT3 pathway

作　　者：王杏[1] 朱慧明 马欢[1] 王超[1] 张会欣 WANG Xing;ZHU Huiming;MA Huan;WANG Chao;ZHANG Huixin(Key Laboratory of Metabolic Disease,Hebei General Hospital,Shijiazhuang 050051,China;Chemical Engineering Institute,Shijiazhuang University,Shijiazhuang 050035,China)

机构地区：[1]河北省人民医院代谢病重点实验室,石家庄050051 [2]石家庄学院化工学院,石家庄050035

出　　处：《中国免疫学杂志》2025年第3期556-560,共5页Chinese Journal of Immunology

基　　金：河北省中医药科研计划项目(2023005);石家庄学院博士科研启动基金(22BS002)。

摘　　要：目的:研究苦参碱对TNF-α诱导的人脐静脉内皮细胞(HUVEC)增殖的影响,探讨其机制是否与调控miR-125b-5p和STAT3表达有关。方法:TNF-α诱导建立HUVEC增殖模型,苦参碱作用后,实时荧光定量PCR检测miR-125b-5p水平,实时荧光定量PCR和免疫印迹法检测增殖细胞核抗原(PCNA)、细胞周期蛋白D1(CyclinD1)、基质金属蛋白酶2(MMP2)、MMP9、STAT3水平。双荧光素酶报告基因实验验证miR-125b-5p与STAT3的靶向关系。结果:苦参碱能抑制TNF-α诱导的HUVEC增殖(P<0.05),降低PCNA、CyclinD1、MMP2、MMP9、STAT3表达(P<0.05),提高miR-125b-5p表达(P<0.05);miR-125b-5p过表达可降低细胞增殖、PCNA、CyclinD1、MMP2、MMP9表达(P<0.05);miR-125b-5p在HUVEC中靶向负调控STAT3表达,抑制miR-125b-5p表达可逆转苦参碱对TNF-α诱导的细胞增殖及PCNA、CyclinD1、MMP2、MMP9、STAT3表达的影响。结论:苦参碱可抑制TNF-α诱导的HUVEC增殖,与调控miR-125b-5p/STAT3途径有关。Objective:To study effect of matrine on TNF-αinduced proliferation of human umbilical vein endothelial cells(HUVEC),as well as to explore whether its mechanism is related to regulation of miR-125b-5p and STAT3 expressions.Methods:HUVEC proliferation model was established by TNF-αstimulating.After matrine treatment,miR-125b-5p level was detected by realtime fluorescent quantitative PCR,proliferating cell nuclear antigen(PCNA),cell cycle protein D1(CyclinD1),matrix metalloproteinase 2(MMP2),MMP9,STAT3 levels were detected by real-time fluorescent quantitative PCR and Western blot.Targeting relationship between miR-125b-5p and STAT3 was verified by dual luciferase reporter gene assay.Results:Matrine inhibited TNF-αinduced HUVEC proliferation(P<0.05),decreased PCNA,CyclinD1,MMP2,MMP9,STAT3 expressions(P<0.05),and increased miR-125b-5p expression(P<0.05).Overexpression of miR-125b-5p could reduce cell proliferation and PCNA,CyclinD1,MMP2,MMP9 expressions HUVEC induced by TNF-α(P<0.05).miR-125b-5p targeted STAT3 expression negatively in HUVEC,and inhibiting miR-125b-5p expression could reverse effect of matrine on TNF-αinduced cell proliferation and PCNA,CyclinD1,MMP2,MMP9 and STAT3 expressions.Conclusion:Matrine inhibits TNF-αinduced proliferation of HUVEC,which is related to regulation of miR-125b-5p/STAT3 pathway.

关 键 词：苦参碱 人脐静脉内皮细胞 增殖 miR-125b-5p STAT3 

分 类 号：R543[医药卫生—心血管疾病]