2型固有淋巴细胞(ILC2)在布鲁氏菌感染中的变化特点研究  

Study on changes of type 2 intrinsic lymphocyte(ILC2)in Brucella infection

作　　者：殷郑伟 朱玥洁[1] 史娟 何月月 丁剑冰[1] 王泉 张峰波[1] YIN Zhengwei;ZHU Yuejie;SHI Juan;HE Yueyue;DING Jianbing;WANG Quan;ZHANG Fengbo(Laboratory of Pathogenesis,Prevention and Treatment of High Incidence Diseases in Central Asia,the First Affiliated Hospital,Xinjiang Medical University,Urumqi 830011,China;Xinjiang Medical University,Collage of Basic Medicine,Urumqi 830011,China;The Eighth Affiliated Hospital,Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]省部共建中亚高发病成因与防治国家重点实验室,新疆医科大学第一附属医院,乌鲁木齐830011 [2]新疆医科大学基础医学院,乌鲁木齐830011 [3]新疆医科大学第八附属医院,乌鲁木齐830011

出　　处：《中国免疫学杂志》2025年第3期655-660,共6页Chinese Journal of Immunology

基　　金：国家自然科学基金(82360394);新疆维吾尔自治区杰出青年项目(2023D01E12);新疆维吾尔自治区科技援疆计划(2022E02061);新疆维吾尔自治区青年科技拔尖人才计划(2022TSYCCX0112)。

摘　　要：目的:探究2型固有淋巴细胞(ILC2)及其相关因子在布鲁氏菌急慢性感染中的变化特点和相关性,明确ILC2在布鲁氏菌病慢性化中的免疫作用。方法:将43例急性布鲁氏菌病患者和45例慢性布鲁氏菌病患者与49例健康对照进行比较。流式细胞术检测各组ILC2水平;荧光定量PCR检测PBMC中GATA3 mRNA水平;ELISA检测血清中IL-33、ST2、IL-4和IL-13等细胞因子水平;全自动生化分析仪检测ALT和AST水平。结果:慢性布病组ILC2水平、PBMC中GATA3 mRNA水平、血清IL-33、IL-4和IL-13水平均较健康对照组及急性布病组显著升高(P<0.01)。相关性分析显示,ILC2水平与GATA3 mRNA、IL-33、IL-4和IL-13水平均呈正相关,与ST2水平呈负相关。ROC曲线表明ILC2、GATA3、IL-33和ST2等指标均对布病患者严重程度有较好的预测能力(AUC>0.7)。结论:ILC2及其相关因子水平升高与布鲁氏菌病慢性化密切相关,可能在布鲁氏菌感染致病过程中起重要免疫作用。Objective:To investigate variation and correlation of type 2 intrinsic lymphocyte(ILC2)and related factors in acute and chronic brucellosis,to identify immune role of ILC2 in chronic brucellosis.Methods:Forty-three patients with acute brucellosis and 45 patients with chronic brucellosis were compared with 49 healthy controls.ILC2 level in each group was detected by flow cytometry.mRNA level of GATA3 in PBMC was detected by fluorescence quantitative PCR.Serum IL-33,ST2,IL-4 and IL-13 levels were detected by ELISA.ALT and AST levels were measured by fully automatic biochemical analyzer.Results:ILC2 level,GATA3 mRNA in PBMC,serum IL-33,IL-4 and IL-13 levels in chronic brucellosis group were significantly higher than healthy control group and acute brucellosis group(P<0.01).Correlation analysis showed that ILC2 level was positively correlated with GATA3 mRNA,IL-33,IL-4 and IL-13 levels,while negatively correlated with ST2 level.ROC curve suggested that ILC2,GATA3,IL-33 and ST2 had good predictive ability for severity of brucellosis patients(AUC>0.7).Conclusion:Increase of ILC2 and its related factors are closely related to chronic brucella infection,which may play an important immune role in pathogenesis of brucella infection.

关 键 词：布鲁氏菌病 2型固有淋巴细胞 GATA3 IL-33 IL-4 IL-13 

分 类 号：R392.11[医药卫生—免疫学]