单细胞测序分析BCR CDR3受体库的研究概况和进展  

Research overview and progress of single-cell sequencing analysis of BCR CDR3 receptor repertoire

作　　者：朱兰伟 李俊 马龙 姚新生 ZHU Lanwei;LI Jun;MA Long;YAO Xinsheng(Department of Immunology,School of Basic Medicine,Zunyi Medical University,Zunyi 563000,China)

机构地区：[1]遵义医科大学基础医学院免疫学教研室,遵义563000

出　　处：《中国免疫学杂志》2025年第3期743-750,共8页Chinese Journal of Immunology

基　　金：国家自然科学基金(81860300);贵州省高层次创新人才项目([2018]5637)。

摘　　要：B细胞受体(BCR)是B细胞特异性识别和结合抗原的主要分子基础,其互补决定区(CDR3)呈现高度多样性和特异性。利用单细胞免疫组库测序技术可以精确地分析每个B细胞BCR CDR3序列的组成和特征,能更好地理解B细胞分化、发育、应答的过程和机制。单细胞免疫组库测序技术为B细胞免疫相关疾病的发病机制、监测、诊断与治疗提供新的思路和理论基础。本文主要对B细胞BCR CDR3受体库的常用检测方法以及检测平台进行对比分析,并重点概述单细胞测序技术在B细胞BCR CDR3受体库中的应用、研究现状和进展。B cell receptor(BCR)is the main molecular basis for B cell specific recognition and binding of antigen,and its complementarity determining region(CDR3)presents high diversity and specificity.Using single-cell immune repertoire sequencing technology can accurately analyze the composition and characteristics of each B cell BCR CDR3 sequence,and can better understand the process and mechanism of B cell differentiation,development,and response.Single-cell immune repertoire sequencing technology provides new ideas and theoretical basis for the pathogenesis,monitoring,diagnosis and treatment of B-cell immune-related diseases.This paper mainly compares and analyzes the commonly used detection methods and detection platforms of B-cell BCR CDR3 receptor library,and focuses on the application,research status and progress of single-cell sequencing technology in B-cell BCR CDR3 receptor library.

关 键 词：B细胞受体 CDR3受体库 单细胞测序 

分 类 号：R392-3[医药卫生—免疫学]