High density sampling reveals the spatiotemporal characteristics of microbial communities in a full-scale municipal wastewater treatment plant  

作　　者：Zhaoyang Li Liang Zhang Jinghan Li Da Kang Jialin Li Shujun Zhang Xiaoyu Han Bin Ma Yongzhen Peng 

机构地区：[1]National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology,Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering,Beijing University of Technology,Beijing 100124,China [2]Beijing Drainage Group Co.,Ltd.(BDG),Beijing 100022,China [3]College of Environment and Ecology,Institute of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,China

出　　处：《Frontiers of Environmental Science & Engineering》2025年第2期135-149,共15页环境科学与工程前沿(英文)

基　　金：financially supported by the National Natural Science Foundation of China(Grant Nos.52122005 and U23A20675).

摘　　要：Insights into the microbial communities in municipal wastewater treatment plants(WWTPs)are critical for the optimization of biological nutrient removal process.However,our understanding about the spatiotemporal characteristics of the microbial communities in WWTPs remains limited.In the present study,264 samples were collected biweekly from four spatially independent corridors in a typical municipal WWTP.The annual compositional and metagenomic characteristics were investigated based on multiple ecological indicators using statistical tests.The results revealed that the microbial community compositions from the four corridors showed significantly high similarities,as revealed by the statistical analysis at the operational taxonomic unit(OTU)level.Consistent with the OTU level results,the functionality of the microbial communities in the four independent corridors also showed significant similarity.In comparison,the dynamics of the microbial community over the year showed two successional peaks of the microbial communities with the spatial similarity,and this resulted in three alternative stable states of the microbial communities in a calendar year.The microbial communities only drifted in July and November,suggesting an uneven community succession pattern driven by seasonal variation in environmental conditions.The functional characteristics were found to be relatively conservative compared to the microbial community succession,which revealed the decoupling between the composition and functionality of the microbial community in the municipal WWTP.The present study provides an in-depth overview of the microbial communities in a municipal WWTP and will be useful for the establishment of the connection between ecological characteristics and the operational stability of WWTPs.

关 键 词：Activated sludge Microbial community Spatial similarity Successional pattern METAGENOMICS 

分 类 号：X70[环境科学与工程—环境工程]