miR-133a-3p及SAR1A在原因不明习惯性流产中作用及预测价值  

Roles and values of miR-133a-3p and SAR1A to the prediction of unexplained habitual abortion

作　　者：王晓静 王旭 赵志红 刘璐 李丽敏 WANG Xiaojing;WANG Xu;ZHAO Zhihong;LIU Lu;LI Limin(Department of Laboratory,Handan First Hospital,Handan,Hebei 056002,China)

机构地区：[1]邯郸市第一医院检验科,河北邯郸056002

出　　处：《中华实用诊断与治疗杂志》2025年第2期145-150,共6页Journal of Chinese Practical Diagnosis and Therapy

基　　金：河北省医学科学研究课题计划项目(20240441)。

摘　　要：目的 观察原因不明习惯性流产(UHA)孕妇胎盘组织miR-133a-3p、SAR1A表达及与免疫功能的相关性,探讨miR-133a-3p、SAR1A预测UHA的价值。方法 2023年3月—2024年2月邯郸市第一医院诊断为UHA的孕妇67例为UHA组,要求人工流产的健康孕妇48例为对照组。2组采用实时荧光定量PCR法检测胎盘组织miR-133a-3p、SAR1A mRNA相对表达量,采用ELISA法检测胎盘组织上清液炎性因子[白细胞介素(IL)-10、IL-17、肿瘤坏死因子-α(TNF-α)等]水平,采用流式细胞术检测胎盘组织免疫细胞(CD8^(+)T淋巴细胞、Th17细胞、Treg细胞等)百分比,并进行比较。采用Spearman相关法分析UHA孕妇胎盘组织miR-133a-3p、SAR1A mRNA相对表达量与炎性因子水平及免疫细胞百分比的相关性;采用多因素logistic回归分析孕妇发生UHA的影响因素;绘制ROC曲线,评估胎盘组织miR-133a-3p、SAR1A mRNA预测孕妇发生UHA的效能。结果 UHA组胎盘组织miR-133a-3p、SAR1A mRNA相对表达量[1.05(0.93, 1.18)、1.14(1.04, 1.29)],IL-17、TNF-α水平[(20.10±4.35)、(22.70±4.45)ng/L]及CD8+T淋巴细胞百分比、Th17细胞百分比[6.08%(4.72%, 7.47%)、3.01%(2.44%, 3.86%)]均高于对照组[0.57(0.48, 0.63)、0.78(0.62, 0.92)、(14.31±3.54)ng/L、(17.03±5.04)ng/L、4.44%(3.66%, 5.85%)、1.70%(1.42%, 2.01%)](Z=4.211~8.668,t=6.372~7.598,P均<0.05),IL-10水平[(18.74±4.29)ng/L]、Treg细胞百分比[2.71%(2.04%, 3.39%)]均低于对照组[(24.78±4.95)ng/L、4.06%(3.39%, 4.95%)](t=6.990,Z=6.114,P均<0.05)。UHA孕妇胎盘组织miR-133a-3p、SAR1A mRNA相对表达量与IL-17、TNF-α水平及CD8+T淋巴细胞百分比、Th17细胞百分比均呈正相关(r=0.312~0.623,P均<0.05),与IL-10水平、Treg细胞百分比均呈负相关(r=-0.509~-0.424,P均<0.05)。胎盘组织miR-133a-3p、SAR1A mRNA相对表达量(OR=2.765,95%CI:1.784~6.610,P=0.001;OR=3.950,95%CI:1.385~4.281,P=0.007),IL-10、IL-17、TNF-α水平(OR=0.769,95%CI:0.618~0.957,P=0.019;OR=1.561,95%CI:1.071~2.275,P=0.020;OR=1.746,95%CI:1.0Objective To observe the expressions of miR-133a-3p and SAR1A in the placental tissues of pregnant women with unexplained habitual abortion(UHA)and their correlations with immune function,and to explore the values of miR-133a-3p and SAR1A to the prediction of UHA in pregnant women.Methods Sixty-seven pregnant women with UHA in Handan First Hospital from March 2023 to February 2024 were selected as the UHA group,and another 48 healthy pregnant women who required induced abortion were included as the control group.Real-time fluorescence quantitative PCR was used to detect the relative expressions of miR-133a-3p and SAR1A mRNAs in placental tissues in two groups.The inflammatory cytokines[interleukin(IL)-10,IL-17,tumor necrosis factor-α(TNF-α)] in the supernatant and the percentages of immune cells(CD8^(+)T lymphocytes,Th17 cells,Treg cells)were detected with ELISA and flow cytometry,and were compared.Spearman correlation coefficient was used to analyze the correlations of the relative expressions of miR-133a-3p and SAR1A mRNAs with the levels of inflammatory cytokines and the percentages of immune cells in UHA pregnant women.Multivariate logistic regression was used to analyze the influencing factors of UHA.ROC curves were plotted to evaluate the efficiencies of miR-133a-3p and SAR1A mRNAs on predicting UHA.Results The relative expressions of miR-133a-3p and SAR1A mRNAs,the levels of IL-17 and TNF-α,and the percentages of CD8+T lymphocytes and Th17 cells were higher in the UHA group[1.05(0.93,1.18),1.14(1.04,1.29),(20.10±4.35)ng/L,(22.70±4.45)ng/L,6.08%(4.72%,7.47%),3.01%(2.44%,3.86%)]than those in the control group[o.57(0.48,0.63),0.78(0.62,0.92),(14.31±3.54)ng/L,(17.03±5.04)ng/L,4.44%(3.66%,5.85%),1.70%(1.42%,2.01%)](Z=4.211-8.668,t=6.372-7.598;allPvalues<0.05),and the level of IL-10 and the percentage of Treg cells were lower in the UHA group[(18.74±4.29)ng/L,2.71%(2.04%,3.39%)]than those in the control group[(24.78±4.95)ng/L,4.06%(3.39%,4.95%)](t=6.990,Z=6.114;all P values<0.05).In the UHA group,the re

关 键 词：原因不明习惯性流产 miR-133a-3p SAR1A 炎性因子 免疫细胞 

分 类 号：R714.21[医药卫生—妇产科学]